Literature DB >> 19892182

Ion-exchange chromatography.

Alois Jungbauer1, Rainer Hahn.   

Abstract

Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.

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Year:  2009        PMID： 19892182     DOI： 10.1016/S0076-6879(09)63022-6

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  9 in total

Review 1.  Recent Trends in System-Scale Integrative Approaches for Discovering Protective Antigens Against Mycobacterial Pathogens.

Authors:  Aarti Rana; Shweta Thakur; Girish Kumar; Yusuf Akhter
Journal:  Front Genet       Date:  2018-11-27       Impact factor: 4.599

2.  Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins.

Authors:  Jenna K Zalewski; Simone Heber; Joshua H Mo; Keith O'Conor; Jeffrey D Hildebrand; Andrew P VanDemark
Journal:  J Vis Exp       Date:  2017-01-06       Impact factor: 1.355

Review 3.  Next-generation Interactomics: Considerations for the Use of Co-elution to Measure Protein Interaction Networks.

Authors:  Daniela Salas; R Greg Stacey; Mopelola Akinlaja; Leonard J Foster
Journal:  Mol Cell Proteomics       Date:  2019-12-02       Impact factor: 5.911

4.  Use of tandem affinity-buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins.

Authors:  Stella M Lai; Pankajavalli Thirugnanasambantham; Vaishnavi Sidharthan; Andrew S Norris; Jamison D Law; Venkat Gopalan; Vicki H Wysocki
Journal:  Methods Enzymol       Date:  2021-09-23       Impact factor: 1.682

5.  Leucine-Rich, Potent Anti-Bacterial Protein against Vibrio cholerae, Staphylococcus aureus from Solanum trilobatum Leaves.

Authors:  Manohar Radhakrishnan; Malathy Palayam; Ammar B Altemimi; Lakshminarayanan Karthik; Gunasekaran Krishnasamy; Francesco Cacciola; Lakshmanan Govindan
Journal:  Molecules       Date:  2022-02-09       Impact factor: 4.411

Review 6.  Sample preparation techniques for the untargeted LC-MS-based discovery of peptides in complex biological matrices.

Authors:  Inez Finoulst; Martijn Pinkse; William Van Dongen; Peter Verhaert
Journal:  J Biomed Biotechnol       Date:  2011-12-12

7.  Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry.

Authors:  Florian Busch; Zachary L VanAernum; Stella M Lai; Venkat Gopalan; Vicki H Wysocki
Journal:  Biochemistry       Date:  2021-06-08       Impact factor: 3.321

8.  Comparison of Depletion Strategies for the Enrichment of Low-Abundance Proteins in Urine.

Authors:  Szymon Filip; Konstantinos Vougas; Jerome Zoidakis; Agnieszka Latosinska; William Mullen; Goce Spasovski; Harald Mischak; Antonia Vlahou; Joachim Jankowski
Journal:  PLoS One       Date:  2015-07-24       Impact factor: 3.240

Review 9.  Phosphorylation of plant virus proteins: Analysis methods and biological functions.

Authors:  Xinjian Zhuang; Xiao Guo; Tianxiao Gu; Xiaowei Xu; Lang Qin; Kai Xu; Zhen He; Kun Zhang
Journal:  Front Microbiol       Date:  2022-07-26       Impact factor: 6.064
  9 in total

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