OBJECTIVE: Recent reports have demonstrated that mesenchymal stem cells (MSCs) can modulate/suppress immunologic responses through interactions with different immune cells. We performed this study in order to investigate the immunomodulatory effects of MSCs in corneal xenotransplantation. ANIMALS STUDIED: Pig and rat. PROCEDURES: We orthotopically transplanted pig corneas into rats and topically applied allogeneic rat MSCs to the corneas for 2 h immediately after transplantation. Graft survival was clinically assessed using slit-lamp biomicroscopy and the median survival time (MST) was calculated. The rejected grafts were histologically examined using antibodies against CD4, CD8, CD161, and CD68. The expression of IL-2, IL-6, IL-10, and IFN-gamma was also evaluated in the rejected grafts using an enzyme-linked immunosorbent assay. RESULTS: The survival of corneal xenografts was not significantly prolonged by MSC application (MST 10.5 days) compared with the controls (MST 9.67 days) (P = 0.4189). Histologically, the rejected grafts in both groups were massively infiltrated with neutrophils and macrophages. Some CD8+ T cells and rare NK cells were found in the rejected grafts. The levels of IL-6 and IL-10 were significantly increased in the rejected grafts from MSC-treated rats compared with the grafts from MSC-untreated rats. However, the levels of IL-2 and IFN-gamma were not different between the two groups. CONCLUSIONS: Topical application of allogeneic rat MSCs was ineffective in prolonging corneal xenograft survival in a pig-to-rat model.
OBJECTIVE: Recent reports have demonstrated that mesenchymal stem cells (MSCs) can modulate/suppress immunologic responses through interactions with different immune cells. We performed this study in order to investigate the immunomodulatory effects of MSCs in corneal xenotransplantation. ANIMALS STUDIED: Pig and rat. PROCEDURES: We orthotopically transplanted pig corneas into rats and topically applied allogeneic rat MSCs to the corneas for 2 h immediately after transplantation. Graft survival was clinically assessed using slit-lamp biomicroscopy and the median survival time (MST) was calculated. The rejected grafts were histologically examined using antibodies against CD4, CD8, CD161, and CD68. The expression of IL-2, IL-6, IL-10, and IFN-gamma was also evaluated in the rejected grafts using an enzyme-linked immunosorbent assay. RESULTS: The survival of corneal xenografts was not significantly prolonged by MSC application (MST 10.5 days) compared with the controls (MST 9.67 days) (P = 0.4189). Histologically, the rejected grafts in both groups were massively infiltrated with neutrophils and macrophages. Some CD8+ T cells and rare NK cells were found in the rejected grafts. The levels of IL-6 and IL-10 were significantly increased in the rejected grafts from MSC-treated rats compared with the grafts from MSC-untreated rats. However, the levels of IL-2 and IFN-gamma were not different between the two groups. CONCLUSIONS: Topical application of allogeneic rat MSCs was ineffective in prolonging corneal xenograft survival in a pig-to-rat model.
Authors: Sherezade Fuentes-Julián; Francisco Arnalich-Montiel; Laia Jaumandreu; Marina Leal; Alfonso Casado; Ignacio García-Tuñon; Enrique Hernández-Jiménez; Eduardo López-Collazo; Maria P De Miguel Journal: PLoS One Date: 2015-03-02 Impact factor: 3.240
Authors: Maribel Marquina; Javier A Collado; Magdiel Pérez-Cruz; Pablo Fernández-Pernas; Juan Fafián-Labora; Francisco J Blanco; Rafael Máñez; María C Arufe; Cristina Costa Journal: Front Immunol Date: 2017-11-06 Impact factor: 7.561
Authors: Lucas Felipe de Oliveira; Thalles Ramos Almeida; Marcus Paulo Ribeiro Machado; Marilia Beatriz Cuba; Angélica Cristina Alves; Marcos Vinícius da Silva; Virmondes Rodrigues Júnior; Valdo José Dias da Silva Journal: Stem Cells Int Date: 2015-08-02 Impact factor: 5.443