BACKGROUND: The pathogenesis of contrast-induced nephropathy (CIN) remains unclear and is defined by changes in serum creatinine which is not a sensitive biomarker for acute kidney injury. Search for differentially expressed urinary protein or peptide could contribute to further understanding of the disease and may provide new biomarkers. METHODS: This is a small sample research. Urine samples were obtained from patients who underwent percutaneous coronary intervention and were labeled with 3 different fluorescent dyes. After 2-dimensional electrophoresis was run, the differentially regulated spots were picked out and identified by mass spectrometry. Another 31 patients were used as validation group. RESULTS: Among 56 significantly changed spots, mannose-binding lectin (MBL) and MBL-associated serine protease 2 were both significantly upregulated. Compared to the baseline value, urinary MBL was significantly increased in the CIN group (2.08, 1.42-5.72, vs. 1.09, 0.516-1.411; p < 0.01). Postprocedure urinary MBL in the CIN group was also significantly higher than that in the non-CIN group (2.08, 1.42-5.72, vs. 1.057, 0.738-1.885; p < 0.05). CONCLUSION: The studies suggested that MBL may be associated with contrast-induced acute kidney injury. It leads to an attempt to define a new pathogenesis and a novel biomarker for CIN. Copyright 2009 S. Karger AG, Basel.
BACKGROUND: The pathogenesis of contrast-induced nephropathy (CIN) remains unclear and is defined by changes in serum creatinine which is not a sensitive biomarker for acute kidney injury. Search for differentially expressed urinary protein or peptide could contribute to further understanding of the disease and may provide new biomarkers. METHODS: This is a small sample research. Urine samples were obtained from patients who underwent percutaneous coronary intervention and were labeled with 3 different fluorescent dyes. After 2-dimensional electrophoresis was run, the differentially regulated spots were picked out and identified by mass spectrometry. Another 31 patients were used as validation group. RESULTS: Among 56 significantly changed spots, mannose-binding lectin (MBL) and MBL-associated serine protease 2 were both significantly upregulated. Compared to the baseline value, urinary MBL was significantly increased in the CIN group (2.08, 1.42-5.72, vs. 1.09, 0.516-1.411; p < 0.01). Postprocedure urinary MBL in the CIN group was also significantly higher than that in the non-CIN group (2.08, 1.42-5.72, vs. 1.057, 0.738-1.885; p < 0.05). CONCLUSION: The studies suggested that MBL may be associated with contrast-induced acute kidney injury. It leads to an attempt to define a new pathogenesis and a novel biomarker for CIN. Copyright 2009 S. Karger AG, Basel.