Comparison of microleakage in human and bovine substrates using confocal microscopy.

Microleakage in human and bovine teeth was compared. Cavities were prepared on the buccal surface in 20 human and 20 bovine teeth (3 mm x 2 mm in depth). The teeth were divided into 4 groups (n = 10) according to the substrate and adhesive (CLEARFIL SE Bond-CF or Scotchbond 1-SB1). Resin composite (Wave) was applied in two increments, each cured for 30 sec. Specimens were stored in 100% relative humidity at 37 degrees C for 24 hr and submitted to 1,000 thermal cycles, followed by immersion in 0.6% aqueous rhodamine for 48 hr. Specimens were rinsed and sectioned at the center. Microleakage length was measured and the score recorded using the following scale: 0-none, 1-up to enamel junction, 2-up to pulp wall, 3-in pulp wall, 4-beneath pulp wall. Percentage of leakage penetration into the cavity was submitted to an ANOVA and Tukey's test (5%) and the scores submitted to the Kruskal-Wallis and Dunn's multiple comparison tests (5%). When bovine teeth were used, SB1 (87.76%) showed a statistically higher penetration mean than CF (66.22%). When human teeth were used, no difference was found between SB1 (47.35%) and CF (36.01%). When scores were analyzed, SB1 showed no difference to CF. The differences found should be taken into consideration when evaluating adhesive microleakage using bovine teeth.