Literature DB >> 19885939

Example of use of taqman real-time RT-PCR to analyze bacterial gene transcript levels: Haemophilus influenzae.

Jason W Johnston1.   

Abstract

The protocol in this unit describes the steps for gene expression analysis of Haemophilus influenzae in broth culture. Steps for hot acid phenol RNA extraction, RNA cleanup, quantitation, assessment, and preparation for TaqMan qRT-PCR are described. The validation, setup, and analysis of TaqMan qRT-PCR experiments are discussed.

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Year:  2009        PMID： 19885939     DOI： 10.1002/9780471729259.mc01d01s15

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  4 in total

1.  Identification of an ATPase, MsmK, which energizes multiple carbohydrate ABC transporters in Streptococcus pneumoniae.

Authors:  Carolyn Marion; Andrew E Aten; Shireen A Woodiga; Samantha J King
Journal:  Infect Immun       Date:  2011-08-08       Impact factor: 3.441

2.  The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides.

Authors:  Caroline M Linke; Shireen A Woodiga; Dustin J Meyers; Carolyn M Buckwalter; Hussam E Salhi; Samantha J King
Journal:  J Bacteriol       Date:  2012-12-21       Impact factor: 3.490

3.  HrrF is the Fur-regulated small RNA in nontypeable Haemophilus influenzae.

Authors:  Estevan A Santana; Alistair Harrison; Xinjun Zhang; Beth D Baker; Benjamin J Kelly; Peter White; Yunlong Liu; Robert S Munson
Journal:  PLoS One       Date:  2014-08-26       Impact factor: 3.240

4.  Mucin acts as a nutrient source and a signal for the differential expression of genes coding for cellular processes and virulence factors in Acinetobacter baumannii.

Authors:  Emily J Ohneck; Brock A Arivett; Steven E Fiester; Cecily R Wood; Maeva L Metz; Gabriella M Simeone; Luis A Actis
Journal:  PLoS One       Date:  2018-01-08       Impact factor: 3.240
  4 in total

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