Literature DB >> 19885824

Time-lapse live imaging of stem cells in Drosophila testis.

Jun Cheng1, Alan J Hunt.   

Abstract

This unit describes a protocol for time-lapse live-imaging of stem cells in Drosophila testis. Testis tips are dissected from Drosophila, sliced, and transferred to glass-bottom chambers where the stem cells residing in their native microenvironment can be monitored in real time. This protocol, facilitated with various fluorescence-labeled markers, allows dynamic cellular processes in stem cells to be characterized throughout the cell cycle.

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Year:  2009        PMID： 19885824      PMCID： PMC2778854          DOI： 10.1002/9780470151808.sc02e02s11

Source DB:  PubMed          Journal:  Curr Protoc Stem Cell Biol        ISSN： 1938-8969


  24 in total

Review 1.  Out of Eden: stem cells and their niches.

Authors:  F M Watt; B L Hogan
Journal:  Science       Date:  2000-02-25       Impact factor: 47.728

2.  Somatic support cells restrict germline stem cell self-renewal and promote differentiation.

Authors:  A A Kiger; H White-Cooper; M T Fuller
Journal:  Nature       Date:  2000-10-12       Impact factor: 49.962

Review 3.  Asymmetric and symmetric stem-cell divisions in development and cancer.

Authors:  Sean J Morrison; Judith Kimble
Journal:  Nature       Date:  2006-06-29       Impact factor: 49.962

4.  Ageing: from stem to stern.

Authors:  Anne Brunet; Thomas A Rando
Journal:  Nature       Date:  2007-09-20       Impact factor: 49.962

5.  Stem cell self-renewal specified by JAK-STAT activation in response to a support cell cue.

Authors:  A A Kiger; D L Jones; C Schulz; M B Rogers; M T Fuller
Journal:  Science       Date:  2001-12-21       Impact factor: 47.728

6.  Stem cells and cancer: two faces of eve.

Authors:  Michael F Clarke; Margaret Fuller
Journal:  Cell       Date:  2006-03-24       Impact factor: 41.582

7.  Somatic control over the germline stem cell lineage during Drosophila spermatogenesis.

Authors:  J Tran; T J Brenner; S DiNardo
Journal:  Nature       Date:  2000-10-12       Impact factor: 49.962

8.  Zfh-1 controls somatic stem cell self-renewal in the Drosophila testis and nonautonomously influences germline stem cell self-renewal.

Authors:  Judith L Leatherman; Stephen Dinardo
Journal:  Cell Stem Cell       Date:  2008-07-03       Impact factor: 24.633

Review 9.  Stem cells find their niche.

Authors:  A Spradling; D Drummond-Barbosa; T Kai
Journal:  Nature       Date:  2001-11-01       Impact factor: 49.962

Review 10.  Male and female Drosophila germline stem cells: two versions of immortality.

Authors:  Margaret T Fuller; Allan C Spradling
Journal:  Science       Date:  2007-04-20       Impact factor: 47.728
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  7 in total

Review 1.  Shining light on Drosophila oogenesis: live imaging of egg development.

Authors:  Li He; Xiaobo Wang; Denise J Montell
Journal:  Curr Opin Genet Dev       Date:  2011-09-17       Impact factor: 5.578

2.  Cytological analysis of spermatogenesis: live and fixed preparations of Drosophila testes.

Authors:  Poojitha Sitaram; Sarah Grace Hainline; Laura Anne Lee
Journal:  J Vis Exp       Date:  2014-01-20       Impact factor: 1.355

3.  Dynamic interplay of spectrosome and centrosome organelles in asymmetric stem cell divisions.

Authors:  Chi Bang; Jun Cheng
Journal:  PLoS One       Date:  2015-04-07       Impact factor: 3.240

4.  A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage.

Authors:  Zsolt G Venkei; Charlotte P Choi; Suhua Feng; Cuie Chen; Steven E Jacobsen; John K Kim; Yukiko M Yamashita
Journal:  PLoS Genet       Date:  2020-03-13       Impact factor: 5.917

5.  An improved organ explant culture method reveals stem cell lineage dynamics in the adult Drosophila intestine.

Authors:  Marco Marchetti; Chenge Zhang; Bruce A Edgar
Journal:  Elife       Date:  2022-08-25       Impact factor: 8.713

6.  Ex vivo culture of Drosophila pupal testis and single male germ-line cysts: dissection, imaging, and pharmacological treatment.

Authors:  Stefanie M K Gärtner; Christina Rathke; Renate Renkawitz-Pohl; Stephan Awe
Journal:  J Vis Exp       Date:  2014-09-11       Impact factor: 1.355

7.  An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues.

Authors:  Matthew P Bostock; Anadika R Prasad; Rita Chaouni; Alice C Yuen; Rita Sousa-Nunes; Marc Amoyel; Vilaiwan M Fernandes
Journal:  Front Cell Dev Biol       Date:  2020-10-06
  7 in total

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