G-X Xiong1, Y Guan, Y Hong, J-W Zhang, H Guan. 1. Department of Rehabilitation, School of Public Health and Family Medicine, Capital Medical University, Beijing, China. roustar@sina.com
Abstract
STUDY DESIGN: Experimental rat study. OBJECTIVE: To investigate changes in motor unit number estimation (MUNE) value of the medial gastrocnemius (MG) muscle after thoracic spinal cord transection in rats and to correlate the MUNE with hindlimb motor function recovery. SETTING: China Rehabilitation Research Center, Beijing, China. METHODS: Twelve rats were subjected to spinal cord transection or sham surgery and then evaluated by MUNE and the Basso, Beattie and Bresnahan (BBB) behavioral scale 56 days after the surgery. RESULTS: The MUNE values of the MG muscle were significantly decreased from baseline at 14-49 days after surgery. However, they returned to near pre-injury levels after 56 days. Rats recovered progressively from the severely impaired hindlimb motor function induced by spinal cord injury, as indicated by a gradual increase in BBB score during days 3-49 after surgery. However, this behavioral recovery was only partial and reached a plateau on day 49. Finally, there was a U-shape-like correlation between changes in MUNE values and BBB scores after thoracic spinal cord transection. CONCLUSIONS: Time-dependent changes in the functional motor unit number may occur in spinal segments caudal to the transection level, and MUNE could be a useful method to evaluate motor function recovery.
STUDY DESIGN: Experimental rat study. OBJECTIVE: To investigate changes in motor unit number estimation (MUNE) value of the medial gastrocnemius (MG) muscle after thoracic spinal cord transection in rats and to correlate the MUNE with hindlimb motor function recovery. SETTING: China Rehabilitation Research Center, Beijing, China. METHODS: Twelve rats were subjected to spinal cord transection or sham surgery and then evaluated by MUNE and the Basso, Beattie and Bresnahan (BBB) behavioral scale 56 days after the surgery. RESULTS: The MUNE values of the MG muscle were significantly decreased from baseline at 14-49 days after surgery. However, they returned to near pre-injury levels after 56 days. Rats recovered progressively from the severely impaired hindlimb motor function induced by spinal cord injury, as indicated by a gradual increase in BBB score during days 3-49 after surgery. However, this behavioral recovery was only partial and reached a plateau on day 49. Finally, there was a U-shape-like correlation between changes in MUNE values and BBB scores after thoracic spinal cord transection. CONCLUSIONS: Time-dependent changes in the functional motor unit number may occur in spinal segments caudal to the transection level, and MUNE could be a useful method to evaluate motor function recovery.
Authors: Markus E Harrigan; Angela R Filous; Andrew P Tosolini; Renee Morris; Jan M Schwab; W David Arnold Journal: Sci Rep Date: 2019-11-13 Impact factor: 4.379