OBJECTIVES: Memory T cells are hallmark of acquired immunological responses. The relationship of mycobacterial antigen-specific CD4(+) memory T cell subsets with pulmonary tuberculosis was investigated. METHODS: The mycobacterial antigen-specific CD4(+) T cells were detected based on CD154 expression and phenotypes of memory T cell were analyzed by surface staining of CD45RA and CCR7 and flow cytometrical analysis in patients with pulmonary tuberculosis and in tuberculin-positive healthy controls. The association of antigen-specific CD4(+) memory T cell subsets with disease severity and anti-TB treatment was analyzed in patients with pulmonary tuberculosis. RESULTS: Patients with pulmonary tuberculosis had significantly lower frequencies of antigen-specific central memory T cells (T(CM)) (p=0.019) and higher frequencies of effector memory T cells (T(EM)) (p=0.022) compared with tuberculin-positive healthy controls without tuberculosis. Patients with smear/culture positive results showed lower population frequencies of T(CM) and significantly higher frequencies of T(EM) (p=0.015) than those with smear/culture negative results. Treatment of TB patients with standard antibiotic regimens for more than one month led to significantly increased frequencies of T(CM) (p=0.031). CONCLUSIONS: The frequencies of mycobacterial antigen-specific T(CM) and T(EM) are associated with disease severity of pulmonary tuberculosis and T(CM) are associated with short-term effects of anti-TB chemotherapy. Copyright 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved.
OBJECTIVES: Memory T cells are hallmark of acquired immunological responses. The relationship of mycobacterial antigen-specific CD4(+) memory T cell subsets with pulmonary tuberculosis was investigated. METHODS: The mycobacterial antigen-specific CD4(+) T cells were detected based on CD154 expression and phenotypes of memory T cell were analyzed by surface staining of CD45RA and CCR7 and flow cytometrical analysis in patients with pulmonary tuberculosis and in tuberculin-positive healthy controls. The association of antigen-specific CD4(+) memory T cell subsets with disease severity and anti-TB treatment was analyzed in patients with pulmonary tuberculosis. RESULTS:Patients with pulmonary tuberculosis had significantly lower frequencies of antigen-specific central memory T cells (T(CM)) (p=0.019) and higher frequencies of effector memory T cells (T(EM)) (p=0.022) compared with tuberculin-positive healthy controls without tuberculosis. Patients with smear/culture positive results showed lower population frequencies of T(CM) and significantly higher frequencies of T(EM) (p=0.015) than those with smear/culture negative results. Treatment of TBpatients with standard antibiotic regimens for more than one month led to significantly increased frequencies of T(CM) (p=0.031). CONCLUSIONS: The frequencies of mycobacterial antigen-specific T(CM) and T(EM) are associated with disease severity of pulmonary tuberculosis and T(CM) are associated with short-term effects of anti-TB chemotherapy. Copyright 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved.
Authors: A D White; C Sarfas; K West; L S Sibley; A S Wareham; S Clark; M J Dennis; A Williams; P D Marsh; S A Sharpe Journal: Clin Vaccine Immunol Date: 2015-06-24
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