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Literature DB >> 19877641

Ammonium sulfate and MALDI in-source decay: a winning combination for sequencing peptides.

Abstract

In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay (ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Peptides
Ammonium Sulfate

Year: 2009 PMID： 19877641 PMCID： PMC2788107 DOI： 10.1021/ac901518a

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

22 in total

1. Molecular mimicry mediated by MHC class Ib molecules after infection with gram-negative pathogens.

Authors: W F Lo; A S Woods; A DeCloux; R J Cotter; E S Metcalf; M J Soloski
Journal: Nat Med Date: 2000-02 Impact factor: 53.440

2. A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain, and cleavage site.

Authors: A R Welch; A S Woods; L M McNally; R J Cotter; W Gibson
Journal: Proc Natl Acad Sci U S A Date: 1991-12-01 Impact factor: 11.205

3. Identification of a Tap-dependent leader peptide recognized by alloreactive T cells specific for a class Ib antigen.

Authors: C J Aldrich; A DeCloux; A S Woods; R J Cotter; M J Soloski; J Forman
Journal: Cell Date: 1994-11-18 Impact factor: 41.582

4. Sequence-specific fragmentation of matrix-assisted laser-desorbed protein/peptide ions.

Authors: R S Brown; J J Lennon
Journal: Anal Chem Date: 1995-11-01 Impact factor: 6.986

5. Natural ligand of mouse CD1d1: cellular glycosylphosphatidylinositol.

Authors: S Joyce; A S Woods; J W Yewdell; J R Bennink; A D De Silva; A Boesteanu; S P Balk; R J Cotter; R R Brutkiewicz
Journal: Science Date: 1998-03-06 Impact factor: 47.728

6. Identification of a ganglioside recognition domain of tetanus toxin using a novel ganglioside photoaffinity ligand.

Authors: R E Shapiro; C D Specht; B E Collins; A S Woods; R J Cotter; R L Schnaar
Journal: J Biol Chem Date: 1997-11-28 Impact factor: 5.157

7. Simplified high-sensitivity sequencing of a major histocompatibility complex class I-associated immunoreactive peptide using matrix-assisted laser desorption/ionization mass spectrometry.

Authors: A S Woods; A Y Huang; R J Cotter; G R Pasternack; D M Pardoll; E M Jaffee
Journal: Anal Biochem Date: 1995-03-20 Impact factor: 3.365

8. Mass spectrometric sequencing of linear peptides by product-ion analysis in a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption ionization.

Authors: R Kaufmann; B Spengler; F Lützenkirchen
Journal: Rapid Commun Mass Spectrom Date: 1993-10 Impact factor: 2.419

Ammonium sulfate and MALDI in-source decay: a winning combination for sequencing peptides.

1. Molecular mimicry mediated by MHC class Ib molecules after infection with gram-negative pathogens.

2. A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain, and cleavage site.

3. Identification of a Tap-dependent leader peptide recognized by alloreactive T cells specific for a class Ib antigen.

4. Sequence-specific fragmentation of matrix-assisted laser-desorbed protein/peptide ions.

5. Natural ligand of mouse CD1d1: cellular glycosylphosphatidylinositol.

6. Identification of a ganglioside recognition domain of tetanus toxin using a novel ganglioside photoaffinity ligand.

7. Simplified high-sensitivity sequencing of a major histocompatibility complex class I-associated immunoreactive peptide using matrix-assisted laser desorption/ionization mass spectrometry.

8. Mass spectrometric sequencing of linear peptides by product-ion analysis in a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption ionization.

9. A gas-liquid solid phase peptide and protein sequenator.

10. A protein sequenator.

1. Highlighting anatomical sub-structures in rat brain tissue using lipid imaging.

2. In-Source Decay and Pseudo-MS³ of Peptide and Protein Ions Using Liquid AP-MALDI.