G A Rama Raju1, G Jaya Prakash, K Murali Krishna, K Madan. 1. Embryology Division, Krishna IVF Clinic, Zillaparishad Junction, Visakhapatnam, Pincode-530 002, Andhra Pradesh, India. krishnaivf@hotmail.com
Abstract
PURPOSE: The present study was undertaken to evaluate and compare the post thaw survival, implantation and pregnancy rates of vitrified human early cavitating blastocysts with deflated expanded blastocysts. MATERIAL AND METHODS: Supernumerary blastocysts were vitrified in 30% ethylene glycol-dimethyl sulphoxide based solution using cryoloop. Fully expanded blastocysts were deflated by gentle aspiration of the blastocoelic fluid using a micromanipulator until the cavity collapses prior to vitrification. RESULTS: Of the 576 vitrified blastocysts, 545 (94.61%) survived thawing in the early cavitating blastocyst group which was significantly higher than deflated expanded blastocyst group, in which only 370 survived thawing out of 459 (80.62%). However, no significant difference was observed in implantation and pregnancy rates between early cavitating and deflated expanded blastocyst groups. CONCLUSIONS: Early cavitating blastocyst would be the ideal stage for cryopreservation of human blastocysts as it has higher survival rate and avoids additional invasive procedures like deflation of the blastocoele.
PURPOSE: The present study was undertaken to evaluate and compare the post thaw survival, implantation and pregnancy rates of vitrified human early cavitating blastocysts with deflated expanded blastocysts. MATERIAL AND METHODS: Supernumerary blastocysts were vitrified in 30% ethylene glycol-dimethyl sulphoxide based solution using cryoloop. Fully expanded blastocysts were deflated by gentle aspiration of the blastocoelic fluid using a micromanipulator until the cavity collapses prior to vitrification. RESULTS: Of the 576 vitrified blastocysts, 545 (94.61%) survived thawing in the early cavitating blastocyst group which was significantly higher than deflated expanded blastocyst group, in which only 370 survived thawing out of 459 (80.62%). However, no significant difference was observed in implantation and pregnancy rates between early cavitating and deflated expanded blastocyst groups. CONCLUSIONS: Early cavitating blastocyst would be the ideal stage for cryopreservation of humanblastocysts as it has higher survival rate and avoids additional invasive procedures like deflation of the blastocoele.
Authors: Eun Kyung Ryu; Yong Soo Hur; Ji Young Ann; Ja Young Maeng; Miji Park; Jeong Hyun Park; Jung Yoon; San Hyun Yoon; Chang Young Hur; Won Don Lee; Jin Ho Lim Journal: Clin Exp Reprod Med Date: 2012-12-31