| Literature DB >> 19875126 |
Ching-Seng Ang1, Julie Rothacker, Heather Patsiouras, Antony W Burgess, Edouard C Nice.
Abstract
Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC-MS/MS to analyse fecal samples from Apc(Min/+) mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75% of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin, haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples. Copyright (c) 2009 Elsevier B.V. All rights reserved.Entities:
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Year: 2009 PMID： 19875126 DOI： 10.1016/j.chroma.2009.10.007
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759