Nic Gillings1. 1. The PET & Cyclotron Unit and The Lundbeck Centre for Integrated Molecular Brain Imaging, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark. nic@pet.rh.dk
Abstract
INTRODUCTION: Analysis of the radioactive components in plasma taken during positron emission tomography (PET) measurements is often vital for the correct quantification of the PET data. The described high-performance liquid chromatography (HPLC) method has been developed to provide a fast, sensitive and robust method for the measurement of plasma samples from PET studies using [(11)C]-labeled radiopharmaceuticals. METHODS: Unadulterated plasma samples were analyzed directly, following a simple filtration, by the use of a small extraction column, containing a restricted access material, combined with a monolithic analysis column in a column-switching HPLC system. RESULTS: Up to 4 ml of plasma was analyzed by this method within 4.5-7 min in a fully automated process. Because of the rapid analysis, a large number of samples could be analyzed during a 90-min PET scan. The extraction column could be used for analysis of up to 500 ml of plasma before replacement was required. CONCLUSIONS: The described method is fast and robust and the large sample volumes allow for accurate determination of the radioactive components in plasma even at 90 min after injection of a [(11)C]-labeled radiopharmaceutical.
INTRODUCTION: Analysis of the radioactive components in plasma taken during positron emission tomography (PET) measurements is often vital for the correct quantification of the PET data. The described high-performance liquid chromatography (HPLC) method has been developed to provide a fast, sensitive and robust method for the measurement of plasma samples from PET studies using [(11)C]-labeled radiopharmaceuticals. METHODS: Unadulterated plasma samples were analyzed directly, following a simple filtration, by the use of a small extraction column, containing a restricted access material, combined with a monolithic analysis column in a column-switching HPLC system. RESULTS: Up to 4 ml of plasma was analyzed by this method within 4.5-7 min in a fully automated process. Because of the rapid analysis, a large number of samples could be analyzed during a 90-min PET scan. The extraction column could be used for analysis of up to 500 ml of plasma before replacement was required. CONCLUSIONS: The described method is fast and robust and the large sample volumes allow for accurate determination of the radioactive components in plasma even at 90 min after injection of a [(11)C]-labeled radiopharmaceutical.
Authors: Anders Ettrup; Martin Hansen; Martin A Santini; James Paine; Nic Gillings; Mikael Palner; Szabolcs Lehel; Matthias M Herth; Jacob Madsen; Jesper Kristensen; Mikael Begtrup; Gitte M Knudsen Journal: Eur J Nucl Med Mol Imaging Date: 2010-12-21 Impact factor: 9.236
Authors: Anders Ettrup; Sophie da Cunha-Bang; Brenda McMahon; Szabolcs Lehel; Agnete Dyssegaard; Anine W Skibsted; Louise M Jørgensen; Martin Hansen; Anders O Baandrup; Søren Bache; Claus Svarer; Jesper L Kristensen; Nic Gillings; Jacob Madsen; Gitte M Knudsen Journal: J Cereb Blood Flow Metab Date: 2014-04-30 Impact factor: 6.200
Authors: Louise M Jørgensen; Pia Weikop; Jonas Villadsen; Tanel Visnapuu; Anders Ettrup; Hanne D Hansen; Anders O Baandrup; Flemming L Andersen; Carsten R Bjarkam; Carsten Thomsen; Bo Jespersen; Gitte M Knudsen Journal: J Cereb Blood Flow Metab Date: 2016-07-20 Impact factor: 6.200
Authors: Hasan Sari; Kjell Erlandsson; Lisbeth Marner; Ian Law; Henrik B W Larsson; Kris Thielemans; Sébastien Ourselin; Simon Arridge; David Atkinson; Brian F Hutton Journal: EJNMMI Res Date: 2018-07-03 Impact factor: 3.138
Authors: Cornelius K Donat; Henrik H Hansen; Hanne D Hansen; Ronnie C Mease; Andrew G Horti; Martin G Pomper; Elina T L'Estrade; Matthias M Herth; Dan Peters; Gitte M Knudsen; Jens D Mikkelsen Journal: Molecules Date: 2020-03-20 Impact factor: 4.411