Literature DB >> 198750

DNA synthesis by partially purified replicating simian virus 40 chromosomes.

H J Edenberg, M A Waqar, J A Huberman.   

Abstract

We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuing DNA synthesis in vitro in the same manner as two crude subnuclear systems we have previously described (4). This indicates that the enzymes and other proteins required for in vitro DNA synthesis are bound to the replicating chromosomes.

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Year:  1977        PMID： 198750      PMCID： PMC342636          DOI： 10.1093/nar/4.9.3083

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Requirement of a Cytoplasmic Fraction for Synthesis of SV40 Deoxyribonucleic Acid in Isolated Nuclei*.

Authors:  M L DePamphilis; P Berg
Journal:  J Biol Chem       Date:  1975-06-10       Impact factor: 5.157

2.  In vitro polyoma DNA synthesis: requirement for cytoplasmic factors.

Authors:  B Francke; T Hunter
Journal:  J Virol       Date:  1975-01       Impact factor: 5.103

3.  Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.

Authors:  B Otto; P Reichard
Journal:  J Virol       Date:  1975-02       Impact factor: 5.103

4.  Subnuclear systems for synthesis of simian virus 40 DNA in vitro.

Authors:  H J Edenberg; M A Waqar; J A Huberman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

5.  Studies on the mode of segregation of histone nu bodies during replication in HeLa cells.

Authors:  R L Seale
Journal:  Cell       Date:  1976-11       Impact factor: 41.582

6.  Dissociation and reconstitution of the DNA replicase system of HeLa cell nuclei.

Authors:  S Seki; G C Mueller
Journal:  Biochim Biophys Acta       Date:  1976-07-02

7.  Dna synthesis in isolated HeLa nuclei. A system for continuation of replication in vivo.

Authors:  H V Hershey; J F Stieber; G C Mueller
Journal:  Eur J Biochem       Date:  1973-04

Review 8.  Eukaryotic chromosome replication.

Authors:  H J Edenberg; J A Huberman
Journal:  Annu Rev Genet       Date:  1975       Impact factor: 16.830

9.  Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle.

Authors:  C Cremisi; P F Pignatti; O Croissant; M Yaniv
Journal:  J Virol       Date:  1975-01       Impact factor: 5.103

10.  In vitro replication of simian virus 40 DNA in a nucleoprotein complex.

Authors:  R T Su; M L DePamphilis
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205
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  11 in total

1.  Herpes simplex virus DNA synthesis in a partially purified soluble extract from infected cells.

Authors:  P F Pignatti; E Cassai; U Bertazzoni
Journal:  J Virol       Date:  1979-12       Impact factor: 5.103

2.  SV40 viral minichromosome: preferential exposure of the origin of replication as probed by restriction endonucleases.

Authors:  A J Varshavsky; O H Sundin; M J Bohn
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

3.  DNA polymerase alpha is associated with replicating SV40 nucleoprotein complexes.

Authors:  B Otto; E Fanning
Journal:  Nucleic Acids Res       Date:  1978-05       Impact factor: 16.971

4.  Simian virus 40 DNA replication in isolated replicating viral chromosomes.

Authors:  R T Su; M L DePamphilis
Journal:  J Virol       Date:  1978-10       Impact factor: 5.103

5.  Association of simian virus 40 T antigen with simian virus 40 nucleoprotein complexes.

Authors:  K Mann; T Hunter
Journal:  J Virol       Date:  1979-01       Impact factor: 5.103

6.  Origin of DNA replication in papovavirus chromatin is recognized by endogenous endonuclease.

Authors:  W Waldeck; B Föhring; K Chowdhury; P Gruss; G Sauer
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

7.  Two-dimensional analysis of proteins sedimenting with simian virus 40 chromosomes.

Authors:  B I Milavetz; L D Spotila; R Thomas; J A Huberman
Journal:  J Virol       Date:  1980-09       Impact factor: 5.103

8.  Extraction of transcribing SV40 chromosomes in very low salt.

Authors:  H J Edenberg
Journal:  Nucleic Acids Res       Date:  1980-02-11       Impact factor: 16.971

9.  Simian virus 40 DNA replication in vitro.

Authors:  J J Li; T J Kelly
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

10.  In vitro DNA synthesis by an alpha-like DNA polymerase bound to replicating simian virus 40 chromosomes.

Authors:  M A Waqar; M J Evans; J F Burke; Y Tsubota; M J Plummer; J A Huberman
Journal:  J Virol       Date:  1983-10       Impact factor: 5.103
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