OBJECTIVE: To establish the method for determining rhynchophylline and isorhynchophylline in Anshen Yangxue oral liquid. METHODS: Rhynchophylline and isorhynchophylline were determined by HPLC. The chromatographic conditions were composed of Kromasil C18 column (5 microm, 4.6 mm x 250 mm), a mixture of methanol and water (55: 45) as mobile phase with 0.01 mol/L triehthylamine, adjusted with acetic acid to pH 7.5. The UV detection wavelength of rhynchophylline and isorhynchophylline was 254 nm. RESULTS: The average coveries of rhynchophylline and isorhynchophylline were 99.04% and 98.75%, respectively. When rhynchophylline's injection quantity between 0.12 - 1.20 microg and isorhynchophylline's injection quantity between 0.08 -0.80 microg, there were good linear relationships (r1 = 0.9999, r2 = 0.9993). CONCLUSION: This method is simple, sensitive, accurate and can be used for the quality control of Anshen Yangxue oral liquid.
OBJECTIVE: To establish the method for determining rhynchophylline and isorhynchophylline in Anshen Yangxue oral liquid. METHODS:Rhynchophylline and isorhynchophylline were determined by HPLC. The chromatographic conditions were composed of Kromasil C18 column (5 microm, 4.6 mm x 250 mm), a mixture of methanol and water (55: 45) as mobile phase with 0.01 mol/L triehthylamine, adjusted with acetic acid to pH 7.5. The UV detection wavelength of rhynchophylline and isorhynchophylline was 254 nm. RESULTS: The average coveries of rhynchophylline and isorhynchophylline were 99.04% and 98.75%, respectively. When rhynchophylline's injection quantity between 0.12 - 1.20 microg and isorhynchophylline's injection quantity between 0.08 -0.80 microg, there were good linear relationships (r1 = 0.9999, r2 = 0.9993). CONCLUSION: This method is simple, sensitive, accurate and can be used for the quality control of Anshen Yangxue oral liquid.