THE MECHANISM OF THE INHIBITION OF HEMOLYSIS : II. EFFECTS OF pH ON INHIBITION BY PLASMA AND BY CHOLESTEROL SOLS.

The effects of variations of pH, from 5.6 to 8.6, on the lytic activity of saponin, digitonin, and sodium taurocholate, and the effects on the inhibition produced by plasma and by cholesterol sols, are described. The results, so far as they can be analyzed, show that the pH dependence of the inhibition produced by plasma is different from that of the inhibition produced by cholesterol sols, and this leads to the conclusion that a considerable part of the inhibition produced by plasma is due to inhibitory substances other than cholesterol. The results also provide a certain amount of indirect evidence that hemolysis is not due primarily to a reaction of the lysins with the cholesterol of the red cell membrane.