Literature DB >> 19872589

A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL.

R B Howland1, A Bernstein.   

Abstract

1. A method for measuring the O(2) consumption of a single cell is described. The cell is placed in a capillary tube adjacent to a bubble of air. KOH (5 per cent) is drawn in on the opposite side of the air and both ends of the tube are sealed with mineral oil. The decrease in the volume of the gas, representing the O(2) consumed, is followed. 2. The possible errors of the technique are appraised. 3. A single Actinosphaerium eichhornii consumes 0.00113 mm.(3) of O(2) per hour. A single Paramecium caudatum consumes 0.00049 mm.(3) of O(2) per hour. 4. The significance of the results and the limitations of the method are discussed.

Entities:  

Year:  1931        PMID: 19872589      PMCID: PMC2141112          DOI: 10.1085/jgp.14.3.339

Source DB:  PubMed          Journal:  J Gen Physiol        ISSN: 0022-1295            Impact factor:   4.086


  4 in total

1.  Rate of respiration in relation to autogamy in Paramecium aurelia.

Authors:  C R PRINGLE; J M STEWART
Journal:  Experientia       Date:  1961-02-15

2.  Respiration rates in heterotrophic, free-living protozoa.

Authors:  T Fenchel; B J Finlay
Journal:  Microb Ecol       Date:  1983-07       Impact factor: 4.552

3.  Editorial: Single Cell Analysis - Discovery, Development and Implications to Study Cell-Cell and Cell-Pathogen Interactions.

Authors:  Weimin Gao; Deepa Rana Jamwal; Jiangxin Wang; Hua Xu
Journal:  Front Cell Dev Biol       Date:  2022-06-22

4.  A microwell array device capable of measuring single-cell oxygen consumption rates.

Authors:  Timothy W Molter; Sarah C McQuaide; Martin T Suchorolski; Tim J Strovas; Lloyd W Burgess; Deirdre R Meldrum; Mary E Lidstrom
Journal:  Sens Actuators B Chem       Date:  2009-01-15       Impact factor: 7.460

  4 in total

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