| Literature DB >> 19869771 |
Abstract
A simple, rapid method for staining all the supravitally stainable cells in the body, as in supravital preparations with neutral red, is described, together with a method for faithful preservation of the dye in paraffin sections. The essential points of the technique are, first, to secure the reaction of cells to neutral red which corresponds to the so-called supravital technique, involving the reaction of only those substances which respond to the dye while the cell is living; and second, to preserve the stain through the processes of fixation, embedding, and counterstaining.Entities:
Year: 1930 PMID： 19869771 PMCID： PMC2131880 DOI： 10.1084/jem.52.3.379
Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307