Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS.

Literature DB >> 19868005

A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS.

Abstract

Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new plates has occurred the digestion and plating can be repeated. The limits of the method have not yet been reached. We are at work on a number of the problems which it has opened up.

Year: 1916 PMID： 19868005 PMCID： PMC2125431 DOI： 10.1084/jem.23.4.549

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

2 in total

1. THE EFFECT OF DILUTION OF PLASMA MEDIUM ON THE GROWTH AND FAT ACCUMULATION OF CELLS IN TISSUE CULTURES.

Authors: R A Lambert
Journal: J Exp Med Date: 1914-04-01 Impact factor: 14.307

2. THE PRESERVATION OF LIVING RED BLOOD CELLS IN VITRO : I. METHODS OF PRESERVATION.

Authors: P Rous; J R Turner
Journal: J Exp Med Date: 1916-02-01 Impact factor: 14.307

2 in total

28 in total

1. Use of the local anesthetic lidocaine for cell harvesting and subcultivation.

Authors: M Rabinovitch; M J DeStefano
Journal: In Vitro Date: 1975 Nov-Dec

2. Modern approaches to the study of mammalian cells in culture.

Authors: H MOSER
Journal: Experientia Date: 1960-09-15

3. [Oxydative decomposition of some radioactive substrates by tumor tissue cultures].

Authors: E BRODA; O HOFFMANN-OSTENHOF; H PERSCHKE; G KELLNER; L STOCKINGER
Journal: Z Krebsforsch Date: 1957

Review 4. Cellular transplantation and gene therapy.

Authors: C Ricordi; S T Ildstad; T E Starzl
Journal: Clin Transplant Date: 1993-02 Impact factor: 2.863

5. Amino acid and vitamin requirements in mammalian cultured cells.

Authors: K Yamamoto; A Niwa
Journal: Amino Acids Date: 1993-02 Impact factor: 3.520

Review 6. Cellular transplants.

Authors: C Ricordi; T E Starzl
Journal: Transplant Proc Date: 1991-02 Impact factor: 1.066

A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS.

1. THE EFFECT OF DILUTION OF PLASMA MEDIUM ON THE GROWTH AND FAT ACCUMULATION OF CELLS IN TISSUE CULTURES.

2. THE PRESERVATION OF LIVING RED BLOOD CELLS IN VITRO : I. METHODS OF PRESERVATION.

1. Use of the local anesthetic lidocaine for cell harvesting and subcultivation.

2. Modern approaches to the study of mammalian cells in culture.

3. [Oxydative decomposition of some radioactive substrates by tumor tissue cultures].

Review 4. Cellular transplantation and gene therapy.

5. Amino acid and vitamin requirements in mammalian cultured cells.

Review 6. Cellular transplants.

7. [Antitumor activity of phallolysin from Amanita phalloides (author's transl)].

8. Serologically demonstrable alloantigens of mouse epidermal cells.

9. Adjuvators to the propagation of mouse mammary tumor cells on expanses of subcutaneous tissue.

10. Establishing a stem cell culture laboratory for clinical trials.