THE INFLUENCE OF TEMPERATURE AND FLUID MEDIUM ON THE SURVIVAL OF EMBRYONIC TISSUES IN VITRO.

1. Embryonic chick and rat tissues preserved at temperatures ranging from -7 degrees to +20 degrees C. live longest at about +6 degrees C. The duration of life under the most favorable conditions is less than twenty days. 2. The kind of isotonic medium used,-plasma serum, Ringer solution, or normal salt solution,-does not appreciably influence the period of survival. The quantity of medium in proportion to tissue is similarly without marked effect.