Literature DB >> 19866769

Isolation of nuclei from yeast.

M M Bhargava1, H O Halvorson.   

Abstract

A method for isolation of nuclei from Saccharomyces cervisiae in high yield is described. The DNA/protein ratio of the isolated nuclei is 10 times higher than that of whole cells. Examination of these nuclei in phase and electron microscopes has shown them to be round bodies having a double membrane, microtubules, and a dark crescent at one end. The optimum conditions for extraction and resolution of histones of these nuclei on acrylamide gels have been investigated. The nuclei have an active RNA polymerase (E.C. 2.7.7.6) and are able to synthesize RNA in vitro. They are also readily stainable with Giemsa's, Feulgen's, and acridine orange methods.

Entities:  

Year:  1971        PMID： 19866769      PMCID： PMC2108327          DOI： 10.1083/jcb.49.2.423

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  17 in total

1.  ON THE DENATURATION OF CHROMOSOMAL DNA IN SITU.

Authors:  D NASH; W PLAUT
Journal:  Proc Natl Acad Sci U S A       Date:  1964-05       Impact factor: 11.205

2.  STUDIES ON THE YEAST NUCLEUS. I. THE ISOLATION OF NUCLEI.

Authors:  T H ROZIJN; G J TONINO
Journal:  Biochim Biophys Acta       Date:  1964-09-11

3.  STUDIES ON ACIDIC NUCLEAR PROTEINS OF THE WALKER TUMOR AND LIVER.

Authors:  W J STEELE; H BUSCH
Journal:  Cancer Res       Date:  1963-09       Impact factor: 12.701

4.  The chemistry of the cell nucleus.

Authors:  V G ALLFREY; A E MIRSKY; H STERN
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1955

5.  New press for disruption of microorganisms.

Authors:  N R EATON
Journal:  J Bacteriol       Date:  1962-06       Impact factor: 3.490

6.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

7.  Identification and estimation of pentoses in the presence of glucose.

Authors:  H F DRURY
Journal:  Arch Biochem       Date:  1948-12

8.  Electron microscopical studies of frozendried yeast. II. The nature of basophile particles and vesicular nuclei in Saccharomyces.

Authors:  B MUNDKUR
Journal:  Exp Cell Res       Date:  1961-10       Impact factor: 3.905

9.  Biochemical and electron microscopic study of isolated yeast nuclei.

Authors:  I Molenaar; W W Sillevis Smitt; T H Rozijn; G J Tonino
Journal:  Exp Cell Res       Date:  1970-05       Impact factor: 3.905

10.  Mitosis in the yeast Lipomyces lipofer.

Authors:  C F ROBINOW
Journal:  J Biophys Biochem Cytol       Date:  1961-04
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  6 in total

1.  Rapid isolation of yeast nuclei.

Authors:  G J Ide; C A Saunders
Journal:  Curr Genet       Date:  1981-11       Impact factor: 3.886

2.  A method for the isolation of nuclei from the dermatophytic fungus, Microsporum gypseum.

Authors:  B C Dill; J J Stock
Journal:  Arch Mikrobiol       Date:  1974-03-28

3.  Low Km cyclic AMP phosphodiesterase of yeast may be bound to ribosomes associated with the nucleus.

Authors:  J Londesborough; L Jönkkäri
Journal:  Mol Cell Biochem       Date:  1982-07-23       Impact factor: 3.396

4.  Balance of osmotic pressures determines the nuclear-to-cytoplasmic volume ratio of the cell.

Authors:  Dan Deviri; Samuel A Safran
Journal:  Proc Natl Acad Sci U S A       Date:  2022-05-17       Impact factor: 12.779

5.  Yeast chromatin: search for histone H1.

Authors: 
Journal:  Mol Gen Genet       Date:  1978-05-31

6.  Formation and fate of cross-links induced by polyfunctional anticancer drugs in yeast.

Authors:  R Fleer; M Brendel
Journal:  Mol Gen Genet       Date:  1979-10-02
  6 in total

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