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Literature DB >> 19866738

Purification of intact microtubules from brain.

J B Kirkpatrick¹, L Hyams, V L Thomas, P M Howley.

Abstract

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.

Entities: Chemical Species

Year: 1970 PMID： 19866738 PMCID： PMC2108086 DOI： 10.1083/jcb.47.2.384

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

33 in total

1. Cytoplasmic filaments and tubules.

Authors: M R Adelman; G G Borisy; M L Shelanski; R C Weisenberg; E W Taylor
Journal: Fed Proc Date: 1968 Sep-Oct

2. Reassociation of microtubule protein.

Authors: R E Stephens
Journal: J Mol Biol Date: 1968-04-28 Impact factor: 5.469

3. The colchicine-binding protein of mammalian brain and its relation to microtubules.

Authors: R C Weisenberg; G G Borisy; E W Taylor
Journal: Biochemistry Date: 1968-12 Impact factor: 3.162

4. Sites of synthesis of chloroplast membrane polypeptides in Chlamydomonas reinhardi y-1.

Authors: J K Hoober
Journal: J Biol Chem Date: 1970-09-10 Impact factor: 5.157

5. Thermal fractionation of outer fiber doublet microtubules into A- and B-subfiber components. A- and B-tubulin.

Authors: R E Stephens
Journal: J Mol Biol Date: 1970-02-14 Impact factor: 5.469

6. Vinblastine-induced precipitation of microtubule protein.

Authors: R Marantz; M Ventilla; M Shelanski
Journal: Science Date: 1969-08-01 Impact factor: 47.728

7. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

8. The biochemical events of mitosis. II. The in vivo and in vitro binding of colchicine in grasshopper embryos and its possible relation to inhibition of mitosis.

Authors: L Wilson; M Friedkin
Journal: Biochemistry Date: 1967-10 Impact factor: 3.162

9. Arms and bridges on microtubules in the mitotic apparatus.

Authors: H J Wilson
Journal: J Cell Biol Date: 1969-03 Impact factor: 10.539

10. Electron microscopy of mitosis in amebae. 3. Cold and urea treatments: a basis for tests of direct effects of mitotic inhibitors on microtubule formation.

Authors: L E Roth
Journal: J Cell Biol Date: 1967-07 Impact factor: 10.539

6 in total

1. Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.

Authors: D B Murphy; G G Borisy
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

Review 2. Ultrastructure and function of the spindle apparatus. Microtubules and chromosomes during nuclear division.

Authors: H Fuge
Journal: Protoplasma Date: 1974 Impact factor: 3.356

3. A depolymerizing effect of the luteinizing hormone and follicle stimulating hormone on the neurotubules of the hypothalamus-hypophysial tract in the rat.

Authors: M I Parada; E L Rodriguez-Echandía
Journal: Experientia Date: 1973-02-15