Literature DB >> 19864481

Multiplex PCR-ligation detection reaction assay for simultaneous detection of drug resistance and toxin genes from Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium.

K Granger1, M S Rundell, M R Pingle, R Shatsky, D H Larone, L M Golightly, F Barany, E D Spitzer.   

Abstract

A multiplex PCR-ligation detection reaction (PCR-LDR) assay was developed for rapid detection of methicillin, tetracycline, and vancomycin resistance, as well as toxic shock toxin and Panton-Valentine leukocidin. The assay was tested on 470 positive blood culture bottles containing Staphylococcus aureus or enterococci. PCR-LDR exhibited a sensitivity and specificity of > or = 98% for all components except tetracycline resistance, which had a sensitivity of 94.7%. Rapid and sensitive detection of antimicrobial resistance and virulence genes could help guide therapy and appropriate infection control measures.

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Year:  2009        PMID： 19864481      PMCID： PMC2812281          DOI： 10.1128/JCM.01411-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

Review 1.  Genetic methods for detection of antimicrobial resistance.

Authors:  Arnfinn Sundsfjord; Gunnar S Simonsen; Bjørg C Haldorsen; Håkon Haaheim; Stig-Ove Hjelmevoll; Pia Littauer; Kristin H Dahl
Journal:  APMIS       Date:  2004 Nov-Dec       Impact factor: 3.205

Review 2.  Update on acquired tetracycline resistance genes.

Authors:  Marilyn C Roberts
Journal:  FEMS Microbiol Lett       Date:  2005-04-15       Impact factor: 2.742

3.  Use of real-time polymerase chain reaction for identification of methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles.

Authors:  John Stratidis; Frank J Bia; Stephen C Edberg
Journal:  Diagn Microbiol Infect Dis       Date:  2007-03-26       Impact factor: 2.803

4.  The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland.

Authors:  Angela S Rossney; Anna C Shore; Pamela M Morgan; Margaret M Fitzgibbon; Brian O'Connell; David C Coleman
Journal:  J Clin Microbiol       Date:  2007-06-20       Impact factor: 5.948

5.  Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

Authors:  Maneesh R Pingle; Kathleen Granger; Philip Feinberg; Rebecca Shatsky; Bram Sterling; Mark Rundell; Eric Spitzer; Davise Larone; Linnie Golightly; Francis Barany
Journal:  J Clin Microbiol       Date:  2007-04-11       Impact factor: 5.948

6.  Community-acquired methicillin-resistant Staphylococcus aureus in Madrid, Spain: transcontinental importation and polyclonal emergence of Panton-Valentine leukocidin-positive isolates.

Authors:  Emilia Cercenado; Oscar Cuevas; Mercedes Marín; Emilio Bouza; Pilar Trincado; Teresa Boquete; Belén Padilla; Ana Vindel
Journal:  Diagn Microbiol Infect Dis       Date:  2008-02-15       Impact factor: 2.803

Review 7.  A review of community-associated methicillin-resistant Staphylococcus aureus skin and soft tissue infections.

Authors:  Rachel J Gorwitz
Journal:  Pediatr Infect Dis J       Date:  2008-01       Impact factor: 2.129

Review 8.  Clinical, epidemiological, and laboratory aspects of methicillin-resistant Staphylococcus aureus (MRSA) infections.

Authors:  Elizabeth Palavecino
Journal:  Methods Mol Biol       Date:  2007

9.  StaphPlex system for rapid and simultaneous identification of antibiotic resistance determinants and Panton-Valentine leukocidin detection of staphylococci from positive blood cultures.

Authors:  Yi-Wei Tang; Abdullah Kilic; Qunying Yang; Sigrid K McAllister; Haijing Li; Rebecca S Miller; Melinda McCormac; Karen D Tracy; Charles W Stratton; Jian Han; Brandi Limbago
Journal:  J Clin Microbiol       Date:  2007-04-19       Impact factor: 5.948

10.  Clinical validation of the molecular BD GeneOhm StaphSR assay for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in positive blood cultures.

Authors:  Paul D Stamper; Mian Cai; Tracy Howard; Sharon Speser; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2007-05-23       Impact factor: 5.948
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  2 in total

1.  Staphylococcal toxic shock syndrome: mechanisms and management.

Authors:  Jonathan A Silversides; Emma Lappin; Andrew J Ferguson
Journal:  Curr Infect Dis Rep       Date:  2010-09       Impact factor: 3.725

2.  Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods.

Authors:  A J M Loonen; A R Jansz; H Kreeftenberg; C A Bruggeman; P F G Wolffs; A J C van den Brule
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-10-24       Impact factor: 3.267
  2 in total

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