PURPOSE: The aim of the present study was to investigate the fertilizing capacity of fresh, frozen-thawed and freeze-dried canine spermatozoa. METHODS: After canine spermatozoa were injected into mouse oocytes, the rates of oocyte activation, male pronuclear formation and chromosomal aberrations were investigated. RESULTS: The rates of oocyte activation were comparable (90.6-100%), no matter the sperm type injected. The percentage of male pronuclear formation was higher (P < 0.001) in the freeze-dried spermatozoa (92.3%) than the fresh (61.5%) and frozen-thawed (69.2%) spermatozoa. However, the chromosomal damage in the oocytes injected with freeze-dried spermatozoa was higher (72.9%: P < 0.001) than with fresh (26.9%) and frozen-thawed (21.4%) spermatozoa. CONCLUSIONS: These data indicate using mouse oocytes that freeze-dried canine spermatozoa may potentially fertilize canine oocytes although chromosomal damage is frequently generated.
PURPOSE: The aim of the present study was to investigate the fertilizing capacity of fresh, frozen-thawed and freeze-dried canine spermatozoa. METHODS: After canine spermatozoa were injected into mouse oocytes, the rates of oocyte activation, male pronuclear formation and chromosomal aberrations were investigated. RESULTS: The rates of oocyte activation were comparable (90.6-100%), no matter the sperm type injected. The percentage of male pronuclear formation was higher (P < 0.001) in the freeze-dried spermatozoa (92.3%) than the fresh (61.5%) and frozen-thawed (69.2%) spermatozoa. However, the chromosomal damage in the oocytes injected with freeze-dried spermatozoa was higher (72.9%: P < 0.001) than with fresh (26.9%) and frozen-thawed (21.4%) spermatozoa. CONCLUSIONS: These data indicate using mouse oocytes that freeze-dried canine spermatozoa may potentially fertilize canine oocytes although chromosomal damage is frequently generated.
Authors: Monika A Ward; Takehito Kaneko; Hirokazu Kusakabe; John D Biggers; David G Whittingham; Ryuzo Yanagimachi Journal: Biol Reprod Date: 2003-08-20 Impact factor: 4.285
Authors: Ji-Long Liu; Hirokazu Kusakabe; Ching-Chien Chang; Hiroyuki Suzuki; David W Schmidt; Marina Julian; Robert Pfeffer; Charles L Bormann; X Cindy Tian; Ryuzo Yanagimachi; Xiangzhong Yang Journal: Biol Reprod Date: 2004-02-11 Impact factor: 4.285
Authors: Daniela Bebbere; Amir Arav; Stefano Mario Nieddu; Giovanni Pietro Burrai; Sara Succu; Pasquale Patrizio; Sergio Ledda Journal: Animals (Basel) Date: 2021-11-29 Impact factor: 2.752