PURPOSE: Endothelial cells, grown within gelatin matrices and implanted onto the adventitia of injured vessels, inhibit stenosis in experimental models. To determine if this technology could be adapted for minimally invasive procedures, the authors compared the effects of cells in an implantable sponge to that of an injectable formulation and investigated the importance of delivery site in a stent model. MATERIALS AND METHODS: Stents were implanted in the femoral arteries of 30 pigs. This was followed by perivascular implantation of sponges or injection of particles containing allogeneic endothelial cells. Controls received acellular matrices or nothing. The effects of delivery site were assessed by injecting cellular matrices into or adjacent to the perivascular tissue or into the neighboring muscle. Animals were sacrificed after 28 days. Pre-sacrifice angiograms and tissue sections were evaluated for stenosis. RESULTS: Arteries treated with cellular matrices had a 55%-63% decrease in angiographic stenosis (P < .05) and a 38%-43% reduction in histologic stenoses (P < .05) compared to controls. Intimal area was greatest when cellular matrices were delivered into the muscle (6.35 mm(2) +/- 0.95) rather than into or adjacent to the perivascular tissue (4.05 mm(2) +/- 0.56 and 4.73 mm(2) +/- 0.53, respectively; P < .05). CONCLUSIONS: Perivascular endothelial cell matrices reduced stenosis after stent-induced injury. The effects were not dependent on the formulation but appeared to be dependent on delivery site. Minimally invasive injections of endothelial cell matrices to the adventitia of arteries following peripheral interventions may decrease restenosis rates.
PURPOSE: Endothelial cells, grown within gelatin matrices and implanted onto the adventitia of injured vessels, inhibit stenosis in experimental models. To determine if this technology could be adapted for minimally invasive procedures, the authors compared the effects of cells in an implantable sponge to that of an injectable formulation and investigated the importance of delivery site in a stent model. MATERIALS AND METHODS: Stents were implanted in the femoral arteries of 30 pigs. This was followed by perivascular implantation of sponges or injection of particles containing allogeneic endothelial cells. Controls received acellular matrices or nothing. The effects of delivery site were assessed by injecting cellular matrices into or adjacent to the perivascular tissue or into the neighboring muscle. Animals were sacrificed after 28 days. Pre-sacrifice angiograms and tissue sections were evaluated for stenosis. RESULTS: Arteries treated with cellular matrices had a 55%-63% decrease in angiographic stenosis (P < .05) and a 38%-43% reduction in histologic stenoses (P < .05) compared to controls. Intimal area was greatest when cellular matrices were delivered into the muscle (6.35 mm(2) +/- 0.95) rather than into or adjacent to the perivascular tissue (4.05 mm(2) +/- 0.56 and 4.73 mm(2) +/- 0.53, respectively; P < .05). CONCLUSIONS: Perivascular endothelial cell matrices reduced stenosis after stent-induced injury. The effects were not dependent on the formulation but appeared to be dependent on delivery site. Minimally invasive injections of endothelial cell matrices to the adventitia of arteries following peripheral interventions may decrease restenosis rates.
Authors: Robert S Schwartz; Elazer R Edelman; Andrew Carter; Nicolas Chronos; Campbell Rogers; Keith A Robinson; Ron Waksman; Judah Weinberger; Robert L Wilensky; Donald N Jensen; Bram D Zuckerman; Renu Virmani Journal: Circulation Date: 2002-10-01 Impact factor: 29.690
Authors: Stephan H Duda; Benjamin Pusich; Goetz Richter; Peter Landwehr; Vincent L Oliva; Alexander Tielbeek; Benjamin Wiesinger; Jan Bart Hak; Hans Tielemans; Gerhard Ziemer; Ecatarina Cristea; Alexandra Lansky; Jean P Bérégi Journal: Circulation Date: 2002-09-17 Impact factor: 29.690
Authors: Helen M Nugent; Adam Groothuis; Philip Seifert; J Luis Guerraro; Mark Nedelman; T Mohanakumar; Elazer R Edelman Journal: J Vasc Res Date: 2002 Nov-Dec Impact factor: 1.934
Authors: Michael Saemisch; Markus Nickmann; Lisa Riesinger; Elazer R Edelman; Heiko Methe Journal: J Tissue Eng Regen Med Date: 2017-12-03 Impact factor: 3.963