Cytokine mRNA expression profiles in peripheral blood mononuclear cells from piglets experimentally co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.

Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) significantly impact the swine industry worldwide. Co-infections with these viruses are common and several lines of evidence suggest that both PRRSV and PCV2 modify host immune responses that facilitate infection. This study examined cytokine mRNA expression profiles of peripheral blood mononuclear cells (PBMCs) from piglets experimentally co-infected with PRRSV and PCV2 to define the influence of co-infection on host immunity. PBMCs from infected and control piglets were stimulated with concanavalin A and the IL-2, IL-4, IL-6, IL-10, IL-12p40, IFN-gamma and TNF-alpha mRNA levels were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). PBMCs from PRRSV/PCV2 co-infected piglets had significantly reduced IL-2, IL-4, IL-6, IL-12p40 and IFN-gamma and significantly increased TNF-alpha mRNA levels compared to those of the piglets infected with either PRRSV or PCV2 alone. The IL-10 mRNA levels in all virus-infected groups were significantly up-regulated early during infection. These results suggested that co-infection synergistically suppresses T helper 1 (Th1)-type and Th2-type cytokine production by PBMCs, indicating that co-infection likely compromises cell-mediated and humoral immune responses resulting in increased severity of the diseases in piglets.