| Literature DB >> 19853573 |
I F C Batista1, O H P Ramos, J S Ventura, I L M Junqueira-de-Azevedo, P L Ho, A M Chudzinski-Tavassi.
Abstract
Bioactive compounds of great interest are found in the saliva of hematophagous organisms. While exploring a cDNA library derived from the salivary glands of the tick Amblyomma cajennense, a transcript that codes for a protein with unique structure (containing an N-terminal Kunitz-type domain and a C-terminus with no homology to any annotated sequences) was found. The recombinant mature form of this protein ( approximately 13.5kDa) was produced in Escherichia coli BL21 (DE3), and it was able to inhibit Factor Xa (FXa) and extend global blood clotting times in vitro and ex vivo. Static and dynamic predictions of its tertiary structure indicate regions that may be related to its FXa inhibitor function. Copyright (c) 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 19853573 DOI： 10.1016/j.abb.2009.10.009
Source DB: PubMed Journal: Arch Biochem Biophys ISSN： 0003-9861 Impact factor: 4.013