Literature DB >> 19853489

Usefulness of blood vessels as a DNA source for PCR-based genotyping based on two cases of corpse dismemberment.

Kaori Shintani-Ishida1, Kazuki Harada, Makoto Nakajima, Ken-ichi Yoshida.   

Abstract

The success of PCR-based genotyping of decomposed remains depends on the quality of extracted DNA. Hard tissues and muscles are preferred because of their DNA stability. However, in dismembered corpses the choice of a suitable DNA source is more limited. In short tandem repeat (STR) analysis in two cases of dismembered corpses, we found an advantage of using blood vessels over muscles. To confirm that blood vessels are better for STR typing compared to muscle, we collected nine sets of blood vessels and the adjacent muscle from six other decomposed remains and compared the STR profiles between the blood vessel and muscle samples. Better results for STR typing were obtained in blood vessels. Based on these results, we recommend use of blood vessels as material for PCR-based genotyping in identification of dismembered human remains with heavy postmortem changes.

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Year:  2009        PMID: 19853489     DOI: 10.1016/j.legalmed.2009.09.004

Source DB:  PubMed          Journal:  Leg Med (Tokyo)        ISSN: 1344-6223            Impact factor:   1.376


  2 in total

1.  Genetic identification of highly putrefied bodies using DNA from soft tissues.

Authors:  Thorsten Schwark; Anke Heinrich; Nicole von Wurmb-Schwark
Journal:  Int J Legal Med       Date:  2010-12-18       Impact factor: 2.686

2.  Quality and quantity of extracted deoxyribonucleic Acid (DNA) from preserved soft tissues of putrefied unidentifiable human corpse.

Authors:  Shashank Pooniya; Sanjeev Lalwani; Anupuma Raina; Tabin Millo; Tirath Das Dogra
Journal:  J Lab Physicians       Date:  2014-01
  2 in total

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