Jaromir Sila1, Pavel Sauer, Milan Kolar. 1. Department of Microbiology, Palacky University and University Hospital, Olomouc, Czech Republic.
Abstract
AIMS: Staphylococcus aureus is an important pathogen characterised by its potential to express many virulence factors. Currently, special attention is being paid to methicillin-resistant strains of S. aureus (MRSA). The aim of this study was to compare the prevalence of 13 selected virulence factor genes in methicillin-resistant versus methicillin-susceptible S. aureus (MSSA) isolates and to investigate their accumulation in the same isolate. METHODS: Real-time PCR was used to detect the presence of genes in 200 isolates of S. aureus (100 MRSA and 100 MSSA) from the University Hospital Olomouc collected in 2005-2006. RESULTS: Six out of the 13 monitored genes were detected more frequently in MRSA isolates: sea, seb, seg, sei, sej and eta, coding for the production of the enterotoxins A, B, G, I, J and the exfoliative toxin A. On the other hand, the pvl and tst genes coding for Panton-Valentine leukocidin and TSST-1 were more frequent in MSSA. Statistical analysis (chi-squared test) of the prevalence of virulence factors in the two groups showed a significant difference (P<0.05) in two cases (seg, sei). CONCLUSIONS: A higher prevalence of selected virulence genes was not confirmed in the methicillin-resistant S. aureus group. This indicates no further increase in their threat.
AIMS: Staphylococcus aureus is an important pathogen characterised by its potential to express many virulence factors. Currently, special attention is being paid to methicillin-resistant strains of S. aureus (MRSA). The aim of this study was to compare the prevalence of 13 selected virulence factor genes in methicillin-resistant versus methicillin-susceptible S. aureus (MSSA) isolates and to investigate their accumulation in the same isolate. METHODS: Real-time PCR was used to detect the presence of genes in 200 isolates of S. aureus (100 MRSA and 100 MSSA) from the University Hospital Olomouc collected in 2005-2006. RESULTS: Six out of the 13 monitored genes were detected more frequently in MRSA isolates: sea, seb, seg, sei, sej and eta, coding for the production of the enterotoxins A, B, G, I, J and the exfoliative toxin A. On the other hand, the pvl and tst genes coding for Panton-Valentine leukocidin and TSST-1 were more frequent in MSSA. Statistical analysis (chi-squared test) of the prevalence of virulence factors in the two groups showed a significant difference (P<0.05) in two cases (seg, sei). CONCLUSIONS: A higher prevalence of selected virulence genes was not confirmed in the methicillin-resistant S. aureus group. This indicates no further increase in their threat.
Authors: Paulo V Damasco; Raiane C Chamon; Angélica T L Barbosa; Sérgio da Cunha; José H W Aquino; Fernanda S Cavalcante; Kátia R N Dos Santos Journal: J Clin Microbiol Date: 2011-11-16 Impact factor: 5.948
Authors: N Indrawattana; O Sungkhachat; N Sookrung; M Chongsa-nguan; A Tungtrongchitr; S P Voravuthikunchai; T Kong-ngoen; H Kurazono; W Chaicumpa Journal: Biomed Res Int Date: 2013-08-31 Impact factor: 3.411