Literature DB >> 19851016

Expression, purification and preliminary crystallographic analysis of Pseudomonas aeruginosa RocR protein.

Masayo Kotaka1, Sujit Dutta, Hooi Chen Lee, Mitchell J M Lim, Yeehwa Wong, Feng Rao, Edward P Mitchell, Zhao Xun Liang, Julien Lescar.   

Abstract

Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 A. The crystals belonged to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 118.8, b = 118.8, c = 495.1 A, alpha = beta = 90, gamma = 120 degrees .

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Year:  2009        PMID: 19851016      PMCID: PMC2765895          DOI: 10.1107/S1744309109034526

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  13 in total

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  1 in total

1.  Structural insights into the regulatory mechanism of the response regulator RocR from Pseudomonas aeruginosa in cyclic Di-GMP signaling.

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  1 in total

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