Zhuo Chen1, Wen Gao, Yong-Gui Ma. 1. Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. chenz@tjh.tjmu.edu
Abstract
OBJECTIVE: To evaluate the effect of Jieze No. 1 Gel (JZ1) on the inner environment of vagina through observing its influences on vaginal levels of lactobacillus, glycogen and lactoferrin in mice. METHODS: One hundred female Kunming mice were randomized into 5 groups, the blank control (BC) group, the blank gel (BG) group, and the three JZ1 treated groups. They were treated via vagina with saline, matrix gel, high (2 mg/microL), middle (1 mg/microL) and low (0.5 mg/microL) dose JZ1 respectively in volume of 20 microL, 72 h after being subcutaneously injected with physiological estradiol benzoate (E2) 2 microg. The 24 h and 72 h living lactobacillus in vaginal lavage fluid cultures were counted, the content of glycogen and the expression of lactoferrin in vaginal tissue were measured. RESULTS: No significant difference was found between the BC group and the three JZ1 treated groups in terms of lactobacillus-CFU, glycogen content and lactoferrin expression, and these indices detected at 24 h were not different to those detected at 72 h in the groups treated with various doses of JZ1 respectively (P > 0.05). CONCLUSION: Once application of JZ1 shows no effect on levels of lactobacillus, glycogen, and lactoferrin expression in the vagina of mice.
OBJECTIVE: To evaluate the effect of Jieze No. 1 Gel (JZ1) on the inner environment of vagina through observing its influences on vaginal levels of lactobacillus, glycogen and lactoferrin in mice. METHODS: One hundred female Kunming mice were randomized into 5 groups, the blank control (BC) group, the blank gel (BG) group, and the three JZ1 treated groups. They were treated via vagina with saline, matrix gel, high (2 mg/microL), middle (1 mg/microL) and low (0.5 mg/microL) dose JZ1 respectively in volume of 20 microL, 72 h after being subcutaneously injected with physiological estradiol benzoate (E2) 2 microg. The 24 h and 72 h living lactobacillus in vaginal lavage fluid cultures were counted, the content of glycogen and the expression of lactoferrin in vaginal tissue were measured. RESULTS: No significant difference was found between the BC group and the three JZ1 treated groups in terms of lactobacillus-CFU, glycogen content and lactoferrin expression, and these indices detected at 24 h were not different to those detected at 72 h in the groups treated with various doses of JZ1 respectively (P > 0.05). CONCLUSION: Once application of JZ1 shows no effect on levels of lactobacillus, glycogen, and lactoferrin expression in the vagina of mice.