BACKGROUND: We recently found that CDH3 was frequently demethylated in advanced colorectal carcinomas. This prompted us to examine the demethylation status of the CDH3 gene in gastric carcinomas. MATERIALS AND METHODS: The demethylation status of the CDH3 gene was examined in primary tumors derived from 36 patients with gastric carcinoma using a quantitative methylation-specific PCR (qMSP) and was evaluated the correlation between the demethylation status and the clinicopathological findings. RESULTS: Demethylation of the CDH3 gene was detected in 25 out of the 36 (69%) primary gastric carcinomas, suggesting that the aberrant demethylation of CDH3 is a frequent event in gastric carcinomas. Demethylation of CDH3 was significantly associated with increasing TNM stage (p=0.0261). Moreover, a trend was shown toward infiltration beyond the serosa being associated with demethylation of CDH3 (p=0.0733). CONCLUSION: CDH3 was frequently demethylated in advanced gastric carcinomas.
BACKGROUND: We recently found that CDH3 was frequently demethylated in advanced colorectal carcinomas. This prompted us to examine the demethylation status of the CDH3 gene in gastric carcinomas. MATERIALS AND METHODS: The demethylation status of the CDH3 gene was examined in primary tumors derived from 36 patients with gastric carcinoma using a quantitative methylation-specific PCR (qMSP) and was evaluated the correlation between the demethylation status and the clinicopathological findings. RESULTS: Demethylation of the CDH3 gene was detected in 25 out of the 36 (69%) primary gastric carcinomas, suggesting that the aberrant demethylation of CDH3 is a frequent event in gastric carcinomas. Demethylation of CDH3 was significantly associated with increasing TNM stage (p=0.0261). Moreover, a trend was shown toward infiltration beyond the serosa being associated with demethylation of CDH3 (p=0.0733). CONCLUSION:CDH3 was frequently demethylated in advanced gastric carcinomas.
Authors: Athanasios Koulis; Natasha Di Costanzo; Catherine Mitchell; Stephen Lade; David Goode; Rita A Busuttil; Alex Boussioutas Journal: BMC Gastroenterol Date: 2022-04-21 Impact factor: 2.847
Authors: Felix Royo; Patricia Zuñiga-Garcia; Verónica Torrano; Ana Loizaga; Pilar Sanchez-Mosquera; Aitziber Ugalde-Olano; Esperanza González; Ana R Cortazar; Laura Palomo; Sonia Fernández-Ruiz; Isabel Lacasa-Viscasillas; Maria Berdasco; James D Sutherland; Rosa Barrio; Amaia Zabala-Letona; Natalia Martín-Martín; Amaia Arruabarrena-Aristorena; Lorea Valcarcel-Jimenez; Alfredo Caro-Maldonado; Jorge Gonzalez-Tampan; Guido Cachi-Fuentes; Manel Esteller; Ana M Aransay; Miguel Unda; Juan M Falcón-Pérez; Arkaitz Carracedo Journal: Oncotarget Date: 2016-02-09