Literature DB >> 19846635

Comparison of PCR and culture for screening of vancomycin-resistant Enterococci: highly disparate results for vanA and vanB.

Anton Mak1, Mark A Miller, George Chong, Yury Monczak.   

Abstract

We compared PCR to conventional culture for the detection of vancomycin-resistant enterococci (VRE) in 30,835 rectal samples over a 3-year period. The positive and negative predictive values of vanB PCR were 1.42% and 99.9%, respectively. A positive vanB result by PCR is poorly predictive and necessitates culture for differentiation of VRE-positive and -negative individuals.

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Year:  2009        PMID： 19846635      PMCID： PMC2786620          DOI： 10.1128/JCM.01547-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli.

Authors:  S A Ballard; E A Grabsch; P D R Johnson; M L Grayson
Journal:  Antimicrob Agents Chemother       Date:  2005-01       Impact factor: 5.191

2.  Direct detection of vanB2 using the Roche LightCycler vanA/B detection assay to indicate vancomycin-resistant enterococcal carriage--sensitive but not specific.

Authors:  H L Young; S A Ballard; P Roffey; M L Grayson
Journal:  J Antimicrob Chemother       Date:  2007-02-06       Impact factor: 5.790

3.  First report of vancomycin-resistant Streptococcus mitis bacteremia in a leukemic patient after prophylaxis with quinolones and during treatment with vancomycin.

Authors:  V Krcmery; S Spanik; J Trupl
Journal:  J Chemother       Date:  1996-08       Impact factor: 1.714

4.  Vancomycin-resistant enterococci: mechanisms and clinical observations.

Authors:  H S Gold
Journal:  Clin Infect Dis       Date:  2001-06-14       Impact factor: 9.079

5.  Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces.

Authors:  M-C Domingo; A Huletsky; A Bernal; R Giroux; D K Boudreau; F J Picard; M G Bergeron
Journal:  J Antimicrob Chemother       Date:  2005-02-24       Impact factor: 5.790

6.  Emergence of vancomycin resistance in the genus Streptococcus: characterization of a vanB transferable determinant in Streptococcus bovis.

Authors:  C Poyart; C Pierre; G Quesne; B Pron; P Berche; P Trieu-Cuot
Journal:  Antimicrob Agents Chemother       Date:  1997-01       Impact factor: 5.191

7.  Molecular characterization of vanB elements in naturally occurring gut anaerobes.

Authors:  S A Ballard; K K Pertile; M Lim; P D R Johnson; M L Grayson
Journal:  Antimicrob Agents Chemother       Date:  2005-05       Impact factor: 5.191

8.  Evaluation of a vanA-specific PCR assay for detection of vancomycin-resistant Enterococcus faecium during a hospital outbreak.

Authors:  M Roger; M C Faucher; P Forest; P St-Antoine; F Coutlée
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

9.  Convenient selective differential broth for isolation of vancomycin-resistant enterococcus from fecal material.

Authors:  Thomas J Novicki; Jeffrey M Schapiro; Bruce K Ulness; Ann Sebeste; Laurel Busse-Johnston; Kristine M Swanson; Susan R Swanzy; Wendy Leisenring; Ajit P Limaye
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

10.  High rates of fecal carriage of nonenterococcal vanB in both children and adults.

Authors:  M Graham; S A Ballard; E A Grabsch; P D R Johnson; M L Grayson
Journal:  Antimicrob Agents Chemother       Date:  2008-01-07       Impact factor: 5.191
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  12 in total

1.  Performance characteristics of the Cepheid Xpert vanA assay for rapid identification of patients at high risk for carriage of vancomycin-resistant Enterococci.

Authors:  N Esther Babady; Kathleen Gilhuley; Diane Cianciminio-Bordelon; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2012-09-12       Impact factor: 5.948

2.  Evaluation of the novel artus C. difficile QS-RGQ, VanR QS-RGQ and MRSA/SA QS-RGQ assays for the laboratory diagnosis of Clostridium difficile infection (CDI), and for vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA) screening.

Authors:  K A Morris; L R Macfarlane-Smith; M H Wilcox
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-12-16       Impact factor: 3.267

3.  Clostridium clostridioforme and Atopobium minutum clinical isolates with vanB-type resistance in France.

Authors:  Jean-Christophe Marvaud; Francine Mory; Thierry Lambert
Journal:  J Clin Microbiol       Date:  2011-07-20       Impact factor: 5.948

Review 4.  Antimicrobial Susceptibility Testing for Enterococci.

Authors:  Ayesha Khan; William R Miller; Dierdre Axell-House; Jose M Munita; Cesar A Arias
Journal:  J Clin Microbiol       Date:  2022-06-13       Impact factor: 11.677

Review 5.  Resistance Mechanisms, Epidemiology, and Approaches to Screening for Vancomycin-Resistant Enterococcus in the Health Care Setting.

Authors:  Matthew L Faron; Nathan A Ledeboer; Blake W Buchan
Journal:  J Clin Microbiol       Date:  2016-05-04       Impact factor: 5.948

6.  Evaluation of the BD GeneOhm MRSA and VanR Assays as a Rapid Screening Tool for Detection of Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococci in a Tertiary Hospital in Saudi Arabia.

Authors:  H Hassan; M Shorman
Journal:  Int J Microbiol       Date:  2011-12-01

7.  Evaluation of the iNtRON VRE vanA/vanB real-time PCR assay for detection of vancomycin-resistant enterococci.

Authors:  Hee Jae Huh; Mi-Ae Jang; Ja Young Seo; Ji-Youn Kim; Chang-Seok Ki; Jong-Won Kim; Nam Yong Lee
Journal:  Ann Lab Med       Date:  2014-12-08       Impact factor: 3.464

8.  Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk.

Authors:  Tsi-Shu Huang; Susan Shin-Jung Lee; Chia-Chien Lee; Chiu-Yen Chen; Fang-Chen Chen; Bao-Chen Chen; Cheng Len Sy; Kuan-Sheng Wu
Journal:  PLoS One       Date:  2017-06-13       Impact factor: 3.240

9.  Screening for vancomycin-resistant enterococci with Xpert® vanA/vanB: diagnostic accuracy and impact on infection control decision making.

Authors:  B J Holzknecht; D S Hansen; L Nielsen; A Kailow; J O Jarløv
Journal:  New Microbes New Infect       Date:  2017-01-12

10.  Genomic insights to control the emergence of vancomycin-resistant enterococci.

Authors:  Benjamin P Howden; Kathryn E Holt; Margaret M C Lam; Torsten Seemann; Susan Ballard; Geoffrey W Coombs; Steven Y C Tong; M Lindsay Grayson; Paul D R Johnson; Timothy P Stinear
Journal:  mBio       Date:  2013-08-13       Impact factor: 7.867
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