Literature DB >> 19844665

Effect of additives on the activity of tannase from Aspergillus awamori MTCC9299.

Vinod Chhokar1, Meenakshi Sangwan, Vikas Beniwal, Kiran Nehra, Kaur S Nehra.   

Abstract

Tannase from Aspergillus awamori MTCC 9299 was purified using ammonium sulfate precipitation followed by ion-exchange chromatography. A purification fold of 19.5 with 13.5% yield was obtained. Temperature of 30 degrees C and pH of 5.5 were found optimum for tannase activity. The effects of metals and organic solvents on the activity of tannase were also studied. Metal ions Mg(+2), Mn(+2), Ca(+2), Na(+), and K(+) stimulated the tannase activity, while Cu(+2), Fe(+3), and Co(+2) acted as inhibitors of the enzyme. The addition of organic solvents like acetic acid, isoamylalcohol, chloroform, isopropyl alcohol, and ethanol completely inhibited the enzyme activity. However, butanol and benzene increased the enzyme activity.

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Year:  2009        PMID： 19844665     DOI： 10.1007/s12010-009-8813-7

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  8 in total

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Journal:  Appl Biochem Biotechnol       Date:  2021-03-09       Impact factor: 2.926

2.  Characterization of Aspergillus fumigatus CAS-21 tannase with potential for propyl gallate synthesis and treatment of tannery effluent from leather industry.

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3.  Enhanced DPPH radical scavenging activity and DNA protection effect of litchi pericarp extract by Aspergillus awamori bioconversion.

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Journal:  Chem Cent J       Date:  2012-09-27       Impact factor: 4.215

4.  Novel strategies for upstream and downstream processing of tannin acyl hydrolase.

Authors:  Luis V Rodríguez-Durán; Blanca Valdivia-Urdiales; Juan C Contreras-Esquivel; Raúl Rodríguez-Herrera; Cristóbal N Aguilar
Journal:  Enzyme Res       Date:  2011-09-19

5.  Response Surface Methodology Based Optimization, Partial Purification and Characterization of Alkaline Phosphatase Isolated from Pseudomonas asiatica Strain ZKB1 and its Application in Plant Growth Promotion.

Authors:  Zabin K Bagewadi; Deepak A Yaraguppi; Sikandar I Mulla; Sanjay H Deshpande
Journal:  Mol Biotechnol       Date:  2022-03-12       Impact factor: 2.860

6.  Purification and characterization of tannin acyl hydrolase produced by mixed solid state fermentation of wheat bran and marigold flower by Penicillium notatum NCIM 923.

Authors:  Saswati Gayen; Uma Ghosh
Journal:  Biomed Res Int       Date:  2013-11-17       Impact factor: 3.411

7.  Expression, purification and immobilization of tannase from Staphylococcus lugdunensis MTCC 3614.

Authors:  Amballa Chaitanyakumar; M Anbalagan
Journal:  AMB Express       Date:  2016-10-04       Impact factor: 3.298

8.  Optimization of chromium and tannic acid bioremediation by Aspergillus niveus using Plackett-Burman design and response surface methodology.

Authors:  Prachi Chaudhary; Vinod Chhokar; Pragati Choudhary; Anil Kumar; Vikas Beniwal
Journal:  AMB Express       Date:  2017-11-14       Impact factor: 3.298
  8 in total

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