Evaluation of PermaNet 2.0 mosquito bednets against mosquitoes, including Anopheles minimus s.l., in India.

Wash resistance and field bioefficacy of PermaNet 2.0 nets, long lasting insecticidal nets, against mosquitoes were evaluated in Assam, northeastern India. After repeated hand washings at 12-day intervals a decline in the mosquito killing ability of PermaNet nets was noted (trend chi2 38.9, p < 0.0001), yet these nets retained good insecticidal efficacy for up to 15 wash cycles, producing a 72.5% mean mortality of An. minimus 24 hours after a 3-minute exposure in World Health Organization cones after 15 washings. Significantly fewer (p < 0.001) mosquitoes were captured in self-baited landing collections in houses equipped with PermaNet nets than in houses using untreated nets in the case of culicines (p < 0.001) but not with anophelines, including An. minimus. The use of PermaNet nets resulted in noticeably fewer bites from Culex pseudovishnui (68.5%) and Cx. quinquefasciatus (70%). Blood-feeding inhibition of mosquitoes in deliberately torn PermaNet nets was seen, indicating a protective effect for those sleeping under these nets. No serious adverse effects of the PermaNet nets were reported by users.