| Literature DB >> 19837043 |
Fernando Lopes Pinto1, Peter Lindblad.
Abstract
Rapid amplification of cDNA ends (RACE) is an established strategy used to determine the transcription start point(s) and the 5' untranslated region(s) of mRNA. Different approaches to perform 5' RACE are available, and one particularly simple and powerful strategy is based on a phenomenon called template-switching. We investigated different aspects of template-switch-based 5' RACE, and we describe the different steps leading to the in-house development of a complete 5' RACE system-from oligonucleotide design to polymerase chain reaction (PCR) amplification. We show that the resulting system is reliable, time-efficient, and inexpensive. Copyright (c) 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 19837043 DOI： 10.1016/j.ab.2009.10.022
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365