Literature DB >> 19835059

Establishing a 3D ex vivo culture system for investigations of bone metabolism and biomaterial interactions.

R Geoff Richards1, Angharad E Simpson, Katharina Jaehn, Pamela I Furlong, Martin J Stoddart.   

Abstract

A 3D load-providing culture system is presented, which enables human cancellous bone explants to be studied ex vivo. Bone integrity and activity was demonstrated during 2 to 3 weeks of culture. Viable osteocytes and bone specific markers could be identified. Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies.

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Year:  2007        PMID： 19835059

Source DB:  PubMed          Journal:  ALTEX        ISSN： 1868-596X            Impact factor:   6.043


  7 in total

1.  A novel three-dimensional bone chip organ culture.

Authors:  Johannes Kuttenberger; Elzbieta Polska; Birgit M Schaefer
Journal:  Clin Oral Investig       Date:  2012-09-08       Impact factor: 3.573

2.  Trabecular bone adaptation to low-magnitude high-frequency loading in microgravity.

Authors:  Antonia Torcasio; Katharina Jähn; Maarten Van Guyse; Pieter Spaepen; Andrea E Tami; Jos Vander Sloten; Martin J Stoddart; G Harry van Lenthe
Journal:  PLoS One       Date:  2014-05-02       Impact factor: 3.240

Review 3.  A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications.

Authors:  Alexander Sieberath; Elena Della Bella; Ana Marina Ferreira; Piergiorgio Gentile; David Eglin; Kenny Dalgarno
Journal:  Int J Mol Sci       Date:  2020-01-30       Impact factor: 5.923

Review 4.  Ex vivo Bone Models and Their Potential in Preclinical Evaluation.

Authors:  E E A Cramer; K Ito; S Hofmann
Journal:  Curr Osteoporos Rep       Date:  2021-01-11       Impact factor: 5.096

5.  The use of rats and mice as animal models in ex vivo bone growth and development studies.

Authors:  A A Abubakar; M M Noordin; T I Azmi; U Kaka; M Y Loqman
Journal:  Bone Joint Res       Date:  2016-12       Impact factor: 5.853

Review 6.  Journey into Bone Models: A Review.

Authors:  Julia Scheinpflug; Moritz Pfeiffenberger; Alexandra Damerau; Franziska Schwarz; Martin Textor; Annemarie Lang; Frank Schulze
Journal:  Genes (Basel)       Date:  2018-05-10       Impact factor: 4.096

7.  Phenotype and Viability of MLO-Y4 Cells Is Maintained by TGFβ₃ in a Serum-Dependent Manner within a 3D-Co-Culture with MG-63 Cells.

Authors:  Katharina Jähn; Deborah J Mason; Jim R Ralphs; Bronwen A J Evans; Charles W Archer; R Geoff Richards; Martin J Stoddart
Journal:  Int J Mol Sci       Date:  2018-06-30       Impact factor: 5.923
  7 in total

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