BACKGROUND: It has been proposed that single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) could affect the expression of the miRNA and contribute to the susceptibility of human tumors. However, the role of genetic variant (T/C) in miR-196a-2 in gastric cancer susceptibility is still unknown. OBJECTIVES: To evaluate the association between genetic polymorphism of miR-196a-2 (rs11614913) and risk of gastric cancer, a hospital-based case-control study was conducted in a Chinese population. METHODS: The miR-196a-2 polymorphism was determined using the method of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) in 213 gastric cancer patients and 213 age- and sex-matched controls. RESULTS: In the present study, we found that a significantly increased risk of gastric cancer in subjects with the variant homozygote CC of miR-196a-2 compared with wild-type homozygote TT and heterozygote CT carriers (adjusted odds ratio (OR) = 1.57, 95% confidence interval (CI) = 1.03-2.39, P = 0.038). Stratified analyses indicated that the variant homozygote CC genotype had a strong association with lymph node metastasis of gastric cancer (adjusted OR = 2.25, 95% CI = 1.21-4.18, P = 0.011). CONCLUSIONS: These findings suggest that the genetic variant within miR-196a-2 could play an important role in the development and progression of gastric cancer. We expect the findings may be helpful to better understand the mechanism of gastric carcinogenesis.
BACKGROUND: It has been proposed that single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) could affect the expression of the miRNA and contribute to the susceptibility of humantumors. However, the role of genetic variant (T/C) in miR-196a-2 in gastric cancer susceptibility is still unknown. OBJECTIVES: To evaluate the association between genetic polymorphism of miR-196a-2 (rs11614913) and risk of gastric cancer, a hospital-based case-control study was conducted in a Chinese population. METHODS: The miR-196a-2 polymorphism was determined using the method of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) in 213 gastric cancerpatients and 213 age- and sex-matched controls. RESULTS: In the present study, we found that a significantly increased risk of gastric cancer in subjects with the variant homozygote CC of miR-196a-2 compared with wild-type homozygote TT and heterozygote CT carriers (adjusted odds ratio (OR) = 1.57, 95% confidence interval (CI) = 1.03-2.39, P = 0.038). Stratified analyses indicated that the variant homozygote CC genotype had a strong association with lymph node metastasis of gastric cancer (adjusted OR = 2.25, 95% CI = 1.21-4.18, P = 0.011). CONCLUSIONS: These findings suggest that the genetic variant within miR-196a-2 could play an important role in the development and progression of gastric cancer. We expect the findings may be helpful to better understand the mechanism of gastric carcinogenesis.
Authors: Stefano Volinia; George A Calin; Chang-Gong Liu; Stefan Ambs; Amelia Cimmino; Fabio Petrocca; Rosa Visone; Marilena Iorio; Claudia Roldo; Manuela Ferracin; Robyn L Prueitt; Nozumu Yanaihara; Giovanni Lanza; Aldo Scarpa; Andrea Vecchione; Massimo Negrini; Curtis C Harris; Carlo M Croce Journal: Proc Natl Acad Sci U S A Date: 2006-02-03 Impact factor: 11.205
Authors: Francesco Gianfagna; Emma De Feo; Cornelia M van Duijn; Gualtiero Ricciardi; Stefania Boccia Journal: Curr Genomics Date: 2008-09 Impact factor: 2.236
Authors: Brock C Christensen; Michele Avissar-Whiting; Lauren G Ouellet; Rondi A Butler; Heather H Nelson; Michael D McClean; Carmen J Marsit; Karl T Kelsey Journal: Clin Cancer Res Date: 2010-05-25 Impact factor: 12.531