BACKGROUND: Different studies regarding VZV genotype distribution worldwide have demonstrated that genetic diversity and epidemiology of infection significantly vary from region to region. In Mexico, VZV genotype distribution is largely unknown mostly due to the lack of a surveillance system that monitors accurately the presence of viral strains circulating in the country. OBJECTIVE: To identify the main VZV genotypes circulating in the metropolitan area of Mexico City. STUDY DESIGN: In this study, 127 different VZV isolates, obtained from residents of the Mexico City Metropolitan area from 2005 to 2008, were identified and genotyped. Viral detection and preliminary genotyping was performed by amplification of the VZV ORF-38 and -54 and RFLP analysis using PstI and BglI endonuclease restriction patterns, respectively. Genotype was confirmed by nucleotide sequence variation along the ORF-22. RESULTS: RFLP analysis classified 121 viral strains as European and 6 as mosaic genotype. Genotyping scheme based on the ORF-22 sequence variation identified 120 viral strains belonging to the E genotype, 6 M1 and 1 M4 genotype strains. CONCLUSIONS: VZV European genotype appears to predominate in Mexico City. This is the first study addressing VZV genotype distribution in Mexico. The information reported in this paper may be useful for future epidemiological studies conducted in the country and also contributes to understand better the molecular epidemiology of VZV in the Americas.
BACKGROUND: Different studies regarding VZV genotype distribution worldwide have demonstrated that genetic diversity and epidemiology of infection significantly vary from region to region. In Mexico, VZV genotype distribution is largely unknown mostly due to the lack of a surveillance system that monitors accurately the presence of viral strains circulating in the country. OBJECTIVE: To identify the main VZV genotypes circulating in the metropolitan area of Mexico City. STUDY DESIGN: In this study, 127 different VZV isolates, obtained from residents of the Mexico City Metropolitan area from 2005 to 2008, were identified and genotyped. Viral detection and preliminary genotyping was performed by amplification of the VZV ORF-38 and -54 and RFLP analysis using PstI and BglI endonuclease restriction patterns, respectively. Genotype was confirmed by nucleotide sequence variation along the ORF-22. RESULTS: RFLP analysis classified 121 viral strains as European and 6 as mosaic genotype. Genotyping scheme based on the ORF-22 sequence variation identified 120 viral strains belonging to the E genotype, 6 M1 and 1 M4 genotype strains. CONCLUSIONS: VZV European genotype appears to predominate in Mexico City. This is the first study addressing VZV genotype distribution in Mexico. The information reported in this paper may be useful for future epidemiological studies conducted in the country and also contributes to understand better the molecular epidemiology of VZV in the Americas.
Authors: Gilberto Vaughan; Araceli Rodríguez-Castillo; Mayra Y Cruz-Rivera; Karina Ruiz-Tovar; José E Ramírez-González; Pilar Rivera-Osorio; Salvador Fonseca-Coronado; Juan C Carpio-Pedroza; Fernando Cazares; Mauricio Vazquez-Pichardo; Luis Anaya; Alejandro Escobar-Gutiérrez Journal: Virol J Date: 2011-07-27 Impact factor: 4.099
Authors: Patrick Bryant; Tugba Yildirim; Sara B Griesemer; Kara Shaw; Dylan Ehrbar; Kirsten St George Journal: J Clin Microbiol Date: 2022-03-24 Impact factor: 11.677
Authors: Fabiola Garcés-Ayala; Araceli Rodríguez-Castillo; Joanna María Ortiz-Alcántara; Elizabeth Gonzalez-Durán; José Miguel Segura-Candelas; Sandra Ivette Pérez-Agüeros; Noé Escobar-Escamilla; Alfonso Méndez-Tenorio; José Alberto Diaz-Quiñonez; José Ernesto Ramirez-González Journal: Genome Announc Date: 2015-07-09