GOALS: Assessment of disease severity is a frequent challenge in the management of Crohn's disease. Noninvasive, accurate markers for monitoring disease activity are urgently required. Specific gene expression patterns and molecular biomarkers associated with active Crohn's disease could serve as such markers, thereby providing a novel approach to disease activity monitoring. BACKGROUND: Gene expression profiling in circulating leukocytes has shown promise in several medical conditions and blood may provide an easily accessible surrogate tissue for using gene expression profiling to assess activity of Crohn's disease. STUDY: In this study, we compared genome-wide transcription profiles of circulating leukocytes in patients with active and quiescent Crohn's disease. RESULTS: We observed complex changes in blood gene expression patterns in active Crohn's disease: genes of various functional categories were differentially regulated between active and inactive Crohn's disease. We specifically identified a number of inflammatory molecules overexpressed or underexpressed in active Crohn's disease and validated a subset of these genes by real-time reverse transcription-polymerase chain reaction. CONCLUSIONS: The genes differentially regulated in peripheral leukocytes represent potential new biomarkers for assessing the activity of Crohn's disease.
GOALS: Assessment of disease severity is a frequent challenge in the management of Crohn's disease. Noninvasive, accurate markers for monitoring disease activity are urgently required. Specific gene expression patterns and molecular biomarkers associated with active Crohn's disease could serve as such markers, thereby providing a novel approach to disease activity monitoring. BACKGROUND: Gene expression profiling in circulating leukocytes has shown promise in several medical conditions and blood may provide an easily accessible surrogate tissue for using gene expression profiling to assess activity of Crohn's disease. STUDY: In this study, we compared genome-wide transcription profiles of circulating leukocytes in patients with active and quiescent Crohn's disease. RESULTS: We observed complex changes in blood gene expression patterns in active Crohn's disease: genes of various functional categories were differentially regulated between active and inactive Crohn's disease. We specifically identified a number of inflammatory molecules overexpressed or underexpressed in active Crohn's disease and validated a subset of these genes by real-time reverse transcription-polymerase chain reaction. CONCLUSIONS: The genes differentially regulated in peripheral leukocytes represent potential new biomarkers for assessing the activity of Crohn's disease.
Authors: Jerzy Ostrowski; Michalina Dabrowska; Izabella Lazowska; Agnieszka Paziewska; Aneta Balabas; Anna Kluska; Maria Kulecka; Jakub Karczmarski; Filip Ambrozkiewicz; Magdalena Piatkowska; Krzysztof Goryca; Natalia Zeber-Lubecka; Jaroslaw Kierkus; Piotr Socha; Michal Lodyga; Maria Klopocka; Barbara Iwanczak; Katarzyna Bak-Drabik; Jaroslaw Walkowiak; Piotr Radwan; Urszula Grzybowska-Chlebowczyk; Bartosz Korczowski; Teresa Starzynska; Michal Mikula Journal: J Crohns Colitis Date: 2019-04-26 Impact factor: 9.071
Authors: Adel M Zaatar; Chun Ren Lim; Chin Wei Bong; Michelle Mei Lin Lee; Jian Jiek Ooi; David Suria; Rakesh Raman; Samuel Chao; Hengxuan Yang; Soon Bin Neoh; Choong-Chin Liew Journal: J Exp Clin Cancer Res Date: 2012-09-17