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Literature DB >> 19820141

Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum.

Judith Becker¹, Corinna Klopprogge, Hartwig Schröder, Christoph Wittmann.

Abstract

In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a >40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.

Entities: Chemical Species

Mesh：

Substances：
Bacterial Proteins
Lysine

Year: 2009 PMID： 19820141 PMCID： PMC2794105 DOI： 10.1128/AEM.01942-09

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

19 in total

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Authors: Jens Olaf Krömer; Michel Fritz; Elmar Heinzle; Christoph Wittmann
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2. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.

Authors: P G Peters-Wendisch; B Schiel; V F Wendisch; E Katsoulidis; B Möckel; H Sahm; B J Eikmanns
Journal: J Mol Microbiol Biotechnol Date: 2001-04

3. Improved L-lysine yield with Corynebacterium glutamicum: use of dapA resulting in increased flux combined with growth limitation.

Authors: L Eggeling; S Oberle; H Sahm
Journal: Appl Microbiol Biotechnol Date: 1998-01 Impact factor: 4.813

4. Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources.

Authors: Judith Becker; Corinna Klopprogge; Oskar Zelder; Elmar Heinzle; Christoph Wittmann
Journal: Appl Environ Microbiol Date: 2005-12 Impact factor: 4.792

5. A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum.

Authors: Junko Ohnishi; Ritsuko Katahira; Satoshi Mitsuhashi; Shingo Kakita; Masato Ikeda
Journal: FEMS Microbiol Lett Date: 2005-01-15 Impact factor: 2.742

6. In silico genome-scale reconstruction and validation of the Corynebacterium glutamicum metabolic network.

Authors: Kjeld Raunkjaer Kjeldsen; Jens Nielsen
Journal: Biotechnol Bioeng Date: 2009-02-01 Impact factor: 4.530

7. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum.

Authors: J Kalinowski; J Cremer; B Bachmann; L Eggeling; H Sahm; A Pühler
Journal: Mol Microbiol Date: 1991-05 Impact factor: 3.501

8. Identification and characterization of the last two unknown genes, dapC and dapF, in the succinylase branch of the L-lysine biosynthesis of Corynebacterium glutamicum.

Authors: Michael Hartmann; Andreas Tauch; Lothar Eggeling; Brigitte Bathe; Bettina Möckel; Alfred Pühler; Jörn Kalinowski
Journal: J Biotechnol Date: 2003-09-04 Impact factor: 3.307

9. Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose.

Authors: Patrick Kiefer; Elmar Heinzle; Oskar Zelder; Christoph Wittmann
Journal: Appl Environ Microbiol Date: 2004-01 Impact factor: 4.792

10. Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme.

Authors: B J Eikmanns; D Rittmann; H Sahm
Journal: J Bacteriol Date: 1995-02 Impact factor: 3.490

14 in total

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Review 2. Recent progress in production of amino acid-derived chemicals using Corynebacterium glutamicum.

Authors: Yota Tsuge; Hiroki Matsuzawa
Journal: World J Microbiol Biotechnol Date: 2021-02-11 Impact factor: 3.312

3. Identification and elimination of the competing N-acetyldiaminopentane pathway for improved production of diaminopentane by Corynebacterium glutamicum.

Authors: Stefanie Kind; Weol Kyu Jeong; Hartwig Schröder; Oskar Zelder; Christoph Wittmann
Journal: Appl Environ Microbiol Date: 2010-06-18 Impact factor: 4.792

4. Control of carbon flux to glutamate excretion in Klebsiella pneumoniae: the role of the indigenous plasmid and its encoded isocitrate dehydrogenase.

Authors: Mansi El-Mansi; Francois Trappey; Ewan Clark; Malcolm Campbell
Journal: J Ind Microbiol Biotechnol Date: 2015-09-29 Impact factor: 3.346

5. The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.

Authors: René Bücker; Ann Kathrin Heroven; Judith Becker; Petra Dersch; Christoph Wittmann
Journal: J Biol Chem Date: 2014-08-27 Impact factor: 5.157