Zhi-Bin Shen1, Yong-Qin Yin, Chun-Ping Tang, Chao-Yan Yan, Chao Chen, Li-Bing Guo. 1. Department of Traditional Chinese Medicinal Chemistry, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, People's Republic of China. szb8113@yahoo.com.cn
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The herbal formula Xiangdan injection and its modifications have been used in traditional Chinese medicine for about hundreds years to alleviate pain and promoting blood. AIM: To investigate the anti-hypoxia active fraction of Xiangdan injection. MATERIALS AND METHODS: Xiangdan injection was extracted by ligarine, chloroform, acetic ether, n-butanol and water, represented respectively by A, B, C, D and E. Five extractions were group on the L12(2(5)) orthogonal designed table. NIH mices anti-hypoxia experiment under normal pressure with rats was adopted on the basis of orthogonal design, prediction pharmacodynamics model of TCM prescriptions was established, and the simulation bias was evaluated by combining two scatterplots. The anti-hypoxia active fraction of Xiangdan injection was determined by experiments. RESULTS: The prediction model of TCM prescriptions established in this study can predict the drug actions for different formulas, and PE% <or= 25% was observed in 112 mice (93.3%). CONCLUSION: As indicated in model prediction and experimental confirmation, the pharmacodynamic actions of the 5 formulas are all superior to that of the original formula, and ADE has the best effect. Crown Copyright 2009. Published by Elsevier Ireland Ltd. All rights reserved.
ETHNOPHARMACOLOGICAL RELEVANCE: The herbal formula Xiangdan injection and its modifications have been used in traditional Chinese medicine for about hundreds years to alleviate pain and promoting blood. AIM: To investigate the anti-hypoxia active fraction of Xiangdan injection. MATERIALS AND METHODS: Xiangdan injection was extracted by ligarine, chloroform, acetic ether, n-butanol and water, represented respectively by A, B, C, D and E. Five extractions were group on the L12(2(5)) orthogonal designed table. NIH mices anti-hypoxia experiment under normal pressure with rats was adopted on the basis of orthogonal design, prediction pharmacodynamics model of TCM prescriptions was established, and the simulation bias was evaluated by combining two scatterplots. The anti-hypoxia active fraction of Xiangdan injection was determined by experiments. RESULTS: The prediction model of TCM prescriptions established in this study can predict the drug actions for different formulas, and PE% <or= 25% was observed in 112 mice (93.3%). CONCLUSION: As indicated in model prediction and experimental confirmation, the pharmacodynamic actions of the 5 formulas are all superior to that of the original formula, and ADE has the best effect. Crown Copyright 2009. Published by Elsevier Ireland Ltd. All rights reserved.