| Literature DB >> 19818791 |
Naruhito Otani1, Koichi Baba, Toshiomi Okuno.
Abstract
Herpes zoster is closely related to decreased varicella-zoster virus (VZV)-specific cell-mediated immunity. We validated a new assay for measuring VZV-specific immunity. We cultured the whole blood of healthy subjects with live attenuated VZV vaccine. Cultured supernatants were harvested at 24-h intervals and assayed for interferon-gamma (IFN-gamma) by an enzyme-linked immunosorbent assay (ELISA). The 48-h culture was suitable for estimating IFN-gamma release. IFN-gamma production was stable after standing for at least 4h at room temperature. IFN-gamma production was observed in whole blood from subjects with recent VZV infection, but not in blood from subjects naïve to the virus. Thus, the IFN-gamma release assay may be useful as a new surrogate assay for measuring VZV-specific immunity.Entities:
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Year: 2009 PMID： 19818791 DOI： 10.1016/j.jim.2009.09.010
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303