| Literature DB >> 19818771 |
P Chen1, B Fenet, S Michaud, N Tomczyk, E Véricel, M Lagarde, M Guichardant.
Abstract
Our study aimed to establish the complete structure of the main dihydroxy conjugated triene issued from the lipoxygenation (soybean enzyme) of docosahexaenoic acid, named PDX, an isomer of protectin/neuroprotectin D1 (PD1/NPD1) described by Bazan and Serhan. NMR approaches and other chemical characterization (e.g. GC-MS, HPLC and LC-MS/MS) indicated that PDX is 10(S),17(S)-dihydroxy-docosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid. The use of (18)O(2) and mass spectrometry showed that PDX is a double lipoxygenation product. Its structure differs from PD1, with E,Z,E geometry (PDX) instead of E,E,Z (PD1) and S configuration at carbon 10 instead of R. PDX inhibits human blood platelet aggregation at sub-micromolar concentrations.Entities:
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Year: 2009 PMID： 19818771 DOI： 10.1016/j.febslet.2009.10.004
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124