Literature DB >> 19816932

Design, synthesis, and amplification of DNA pools for in vitro selection.

Bradley Hall1, John M Micheletti, Pooja Satya, Krystal Ogle, Jack Pollard, Andrew D Ellington.   

Abstract

Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions. (c) 2009 by John Wiley & Sons, Inc.

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Year:  2009        PMID： 19816932     DOI： 10.1002/0471142727.mb2402s88

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  9 in total

Review 1.  Analysis of In Vitro Aptamer Selection Parameters.

Authors:  Maureen McKeague; Erin M McConnell; Jose Cruz-Toledo; Elyse D Bernard; Amanda Pach; Emily Mastronardi; Xueru Zhang; Michael Beking; Tariq Francis; Amanda Giamberardino; Ashley Cabecinha; Annamaria Ruscito; Rocio Aranda-Rodriguez; Michel Dumontier; Maria C DeRosa
Journal:  J Mol Evol       Date:  2015-11-03       Impact factor: 2.395

2.  Functional binding of hexanucleotides to 3C protease of hepatitis A virus.

Authors:  Bärbel S Blaum; Winfried Wünsche; Andrew J Benie; Yuri Kusov; Hannelore Peters; Verena Gauss-Müller; Thomas Peters; Georg Sczakiel
Journal:  Nucleic Acids Res       Date:  2011-12-10       Impact factor: 16.971

3.  A general RNA motif for cellular transfection.

Authors:  Maria L B Magalhães; Michelle Byrom; Amy Yan; Linsley Kelly; Na Li; Raquel Furtado; Deborah Palliser; Andrew D Ellington; Matthew Levy
Journal:  Mol Ther       Date:  2012-01-10       Impact factor: 11.454

Review 4.  Key Aspects of Nucleic Acid Library Design for in Vitro Selection.

Authors:  Maria A Vorobyeva; Anna S Davydova; Pavel E Vorobjev; Alya G Venyaminova
Journal:  Int J Mol Sci       Date:  2018-02-05       Impact factor: 5.923

5.  High-throughput sequencing of the T-cell receptor repertoire: pitfalls and opportunities.

Authors:  James M Heather; Mazlina Ismail; Theres Oakes; Benny Chain
Journal:  Brief Bioinform       Date:  2018-07-20       Impact factor: 11.622

6.  A light-responsive RNA aptamer for an azobenzene derivative.

Authors:  Thea S Lotz; Thomas Halbritter; Christoph Kaiser; Martin M Rudolph; Leon Kraus; Florian Groher; Sabrina Steinwand; Josef Wachtveitl; Alexander Heckel; Beatrix Suess
Journal:  Nucleic Acids Res       Date:  2019-02-28       Impact factor: 16.971

7.  Computational analysis of stochastic heterogeneity in PCR amplification efficiency revealed by single molecule barcoding.

Authors:  Katharine Best; Theres Oakes; James M Heather; John Shawe-Taylor; Benny Chain
Journal:  Sci Rep       Date:  2015-10-13       Impact factor: 4.379

8.  Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection.

Authors:  Abdullah Ozer; Brian S White; John T Lis; David Shalloway
Journal:  Nucleic Acids Res       Date:  2013-06-03       Impact factor: 16.971

9.  High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency.

Authors:  Mayumi Takahashi; Xiwei Wu; Michelle Ho; Pritsana Chomchan; John J Rossi; John C Burnett; Jiehua Zhou
Journal:  Sci Rep       Date:  2016-09-22       Impact factor: 4.379
  9 in total

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