BACKGROUND: A putative Streptococcus pneumoniae serotype, 6D, resulting from the introduction of wciN(beta) into serotype 6B has been proposed. METHODS: We studied 98 unique serogroup 6 isolates from Fijian children, two-thirds of whom had received at least 1 dose of 7-valent pneumococcal conjugate vaccine, and 51 invasive isolates from Australian children. We used a polymerase chain reaction (PCR) system that targets both wciN(beta) and the single-nucleotide polymorphism that differentiates serotypes 6A and 6B-wciP584g (6A) and wciP584a (6B). RESULTS: Two (9%) of 22 Australian isolates and 24 (38%) of 64 Fijian isolates previously identified as 6A by the Quellung reaction and wciP584g PCR contained wciN(beta) and were designated as 6C; 14 (41%) of 34 Fijian isolates previously identified as 6B by the Quellung reaction and wciP584a PCR contained wciN(beta) and were designated as the new putative serotype 6D. A significantly smaller proportion of children from whom serotype 6D was isolated (2/14 [14%]) had not received PCV-7, compared with the proportion of those from whom serotype 6B was isolated (11/20 [55%]) (P < .05). CONCLUSION: This is the first report of naturally occurring S. pneumoniae serotype 6D.
RCT Entities:
BACKGROUND: A putative Streptococcus pneumoniae serotype, 6D, resulting from the introduction of wciN(beta) into serotype 6B has been proposed. METHODS: We studied 98 unique serogroup 6 isolates from Fijian children, two-thirds of whom had received at least 1 dose of 7-valent pneumococcal conjugate vaccine, and 51 invasive isolates from Australian children. We used a polymerase chain reaction (PCR) system that targets both wciN(beta) and the single-nucleotide polymorphism that differentiates serotypes 6A and 6B-wciP584g (6A) and wciP584a (6B). RESULTS: Two (9%) of 22 Australian isolates and 24 (38%) of 64 Fijian isolates previously identified as 6A by the Quellung reaction and wciP584g PCR contained wciN(beta) and were designated as 6C; 14 (41%) of 34 Fijian isolates previously identified as 6B by the Quellung reaction and wciP584a PCR contained wciN(beta) and were designated as the new putative serotype 6D. A significantly smaller proportion of children from whom serotype 6D was isolated (2/14 [14%]) had not received PCV-7, compared with the proportion of those from whom serotype 6B was isolated (11/20 [55%]) (P < .05). CONCLUSION: This is the first report of naturally occurring S. pneumoniae serotype 6D.
Authors: Lotta Siira; Tarja Kaijalainen; Lotte Lambertsen; Moon H Nahm; Maija Toropainen; Anni Virolainen Journal: J Clin Microbiol Date: 2012-06-12 Impact factor: 5.948
Authors: Catherine Satzke; Belinda D Ortika; Shahin Oftadeh; Fiona M Russell; Roy M Robins-Browne; E Kim Mulholland; Gwendolyn L Gilbert Journal: J Clin Microbiol Date: 2010-09-01 Impact factor: 5.948
Authors: J M Marimon; M Ercibengoa; M Alonso; G García-Medina; E Pérez-Trallero Journal: Eur J Clin Microbiol Infect Dis Date: 2010-05-14 Impact factor: 3.267
Authors: F M Russell; J R Carapetis; C Satzke; L Tikoduadua; L Waqatakirewa; R Chandra; A Seduadua; S Oftadeh; Y B Cheung; G L Gilbert; E K Mulholland Journal: Clin Vaccine Immunol Date: 2010-10-13