BACKGROUND: Both thrombomodulin (TM) and endothelial protein C receptor (EPCR) are candidate biomarkers of endothelial damage and have a role of anti-thrombotic defense mechanism in vascular structure. In this study, we aimed to investigate soluble EPCR (sEPCR), soluble TM (sTM) and tumor necrosis factor-alpha (TNF-alpha) levels in hemodialysis (HD) and renal transplant (RTx) recipients. METHODS: Twenty-seven RTx recipients and 15 HD patients were recruited to the study. RTx recipients were evaluated before and 3 months after transplantation. Plasma sEPCR, sTM and TNF-alpha levels were measured at baseline and 3 months later in both groups. Moreover, 27 healthy subjects were evaluated regarding sEPCR. RESULTS: At baseline, there was no difference in sTM, sEPCR, TNF-alpha and C-reactive protein (CRP) between the groups. In paired analysis, sEPCR (266 ± 132 to 117 ± 72 ng/ml), sTM (635 ± 165 to 100 ± 41 ng/ml) and TNF-alpha (11.2 ± 4.3 to 6.0 ± 3.5 pg/ml) were significantly decreased in RTx patients (P < 0.0001) at 3 months, while there was no change in the HD group. In the healthy subjects, sEPCR was found to be 79 ± 26 ng/ml at baseline, which was lower than in both groups. CONCLUSIONS: We showed that the recently proposed endothelial damage biomarkers, sTM and sEPCR, are elevated in HD patients and significantly decrease after kidney transplantation.
BACKGROUND: Both thrombomodulin (TM) and endothelial protein C receptor (EPCR) are candidate biomarkers of endothelial damage and have a role of anti-thrombotic defense mechanism in vascular structure. In this study, we aimed to investigate soluble EPCR (sEPCR), soluble TM (sTM) and tumor necrosis factor-alpha (TNF-alpha) levels in hemodialysis (HD) and renal transplant (RTx) recipients. METHODS: Twenty-seven RTx recipients and 15 HDpatients were recruited to the study. RTx recipients were evaluated before and 3 months after transplantation. Plasma sEPCR, sTM and TNF-alpha levels were measured at baseline and 3 months later in both groups. Moreover, 27 healthy subjects were evaluated regarding sEPCR. RESULTS: At baseline, there was no difference in sTM, sEPCR, TNF-alpha and C-reactive protein (CRP) between the groups. In paired analysis, sEPCR (266 ± 132 to 117 ± 72 ng/ml), sTM (635 ± 165 to 100 ± 41 ng/ml) and TNF-alpha (11.2 ± 4.3 to 6.0 ± 3.5 pg/ml) were significantly decreased in RTxpatients (P < 0.0001) at 3 months, while there was no change in the HD group. In the healthy subjects, sEPCR was found to be 79 ± 26 ng/ml at baseline, which was lower than in both groups. CONCLUSIONS: We showed that the recently proposed endothelial damage biomarkers, sTM and sEPCR, are elevated in HDpatients and significantly decrease after kidney transplantation.
Authors: María José Soler; Ofelia María Martínez-Estrada; Josep Maria Puig-Marí; Didac Marco-Feliu; Anna Oliveras; Joan Vila; Marisa Mir; Antonia Orfila; Senén Vilaró; Josep Lloveras Journal: Am J Transplant Date: 2005-09 Impact factor: 8.086
Authors: Santina Cottone; Alessandro Palermo; Francesco Vaccaro; Giuseppe Mulè; Marco Guarneri; Rosalia Arsena; Anna Vadalà; Giovanni Cerasola Journal: Transpl Int Date: 2007-01 Impact factor: 3.782
Authors: Annette von Drygalski; Christian Furlan-Freguia; Wolfram Ruf; John H Griffin; Laurent O Mosnier Journal: Arterioscler Thromb Vasc Biol Date: 2013-02-07 Impact factor: 8.311