| Literature DB >> 19802601 |
Felipe F de Felippes1, Detlef Weigel.
Abstract
Transient assays provide a convenient alternative to stable transformation. For small RNA analysis in plants, the most widely used method, commonly named agroinfiltration, makes use of Agrobacterium tumefaciens to deliver transgenes into leaf cells of Nicotiana benthamiana. Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Agroinfiltration has been used successfully in many different applications, including the analysis of small RNAs. We describe here a protocol for analysis of miRNA processing using agroinfiltration of N. benthamiana leaves.Entities:
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Year: 2010 PMID： 19802601 DOI： 10.1007/978-1-60327-005-2_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745