Literature DB >> 19802600

Purification of Arabidopsis argonaute complexes and associated small RNAs.

Yijun Qi1, Shijun Mi.   

Abstract

Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.

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Year:  2010        PMID： 19802600     DOI： 10.1007/978-1-60327-005-2_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  An importin β protein negatively regulates MicroRNA activity in Arabidopsis.

Authors:  Wei Wang; Ruiqiang Ye; Ying Xin; Xiaofeng Fang; Chunlian Li; Huiqing Shi; Xueping Zhou; Yijun Qi
Journal:  Plant Cell       Date:  2011-10-07       Impact factor: 11.277

2.  The Phaseolus vulgaris miR159a precursor encodes a second differentially expressed microRNA.

Authors:  Cecilia Contreras-Cubas; Fernando A Rabanal; Catalina Arenas-Huertero; Marco A Ortiz; Alejandra A Covarrubias; José Luis Reyes
Journal:  Plant Mol Biol       Date:  2011-11-15       Impact factor: 4.076

3.  TRANSPORTIN1 Promotes the Association of MicroRNA with ARGONAUTE1 in Arabidopsis.

Authors:  Yuwei Cui; Xiaofeng Fang; Yijun Qi
Journal:  Plant Cell       Date:  2016-09-23       Impact factor: 11.277

4.  Intact RNA structurome reveals mRNA structure-mediated regulation of miRNA cleavage in vivo.

Authors:  Minglei Yang; Hugh C Woolfenden; Yueying Zhang; Xiaofeng Fang; Qi Liu; Maria L Vigh; Jitender Cheema; Xiaofei Yang; Matthew Norris; Sha Yu; Alberto Carbonell; Peter Brodersen; Jiawei Wang; Yiliang Ding
Journal:  Nucleic Acids Res       Date:  2020-09-04       Impact factor: 16.971

5.  Plant ARGONAUTE Protein Immunopurification for Pathogen Cross Kingdom Small RNA Analysis.

Authors:  Florian Dunker; Bernhard Lederer; Arne Weiberg
Journal:  Bio Protoc       Date:  2021-02-05

6.  Massive production of small RNAs from a non-coding region of Cauliflower mosaic virus in plant defense and viral counter-defense.

Authors:  Todd Blevins; Rajendran Rajeswaran; Michael Aregger; Basanta K Borah; Mikhail Schepetilnikov; Loïc Baerlocher; Laurent Farinelli; Frederick Meins; Thomas Hohn; Mikhail M Pooggin
Journal:  Nucleic Acids Res       Date:  2011-03-04       Impact factor: 16.971

7.  RIP-seq of BmAgo2-associated small RNAs reveal various types of small non-coding RNAs in the silkworm, Bombyx mori.

Authors:  Zuoming Nie; Fang Zhou; Dan Li; Zhengbing Lv; Jian Chen; Yue Liu; Jianhong Shu; Qing Sheng; Wei Yu; Wenping Zhang; Caiying Jiang; Yuhua Yao; Juming Yao; Yongfeng Jin; Yaozhou Zhang
Journal:  BMC Genomics       Date:  2013-09-28       Impact factor: 3.969

8.  Viral-inducible Argonaute18 confers broad-spectrum virus resistance in rice by sequestering a host microRNA.

Authors:  Jianguo Wu; Zhirui Yang; Yu Wang; Lijia Zheng; Ruiqiang Ye; Yinghua Ji; Shanshan Zhao; Shaoyi Ji; Ruofei Liu; Le Xu; Hong Zheng; Yijun Zhou; Xin Zhang; Xiaofeng Cao; Lianhui Xie; Zujian Wu; Yijun Qi; Yi Li
Journal:  Elife       Date:  2015-02-17       Impact factor: 8.140

9.  The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules.

Authors:  Aarón Barraza; Cecilia Contreras-Cubas; Georgina Estrada-Navarrete; José L Reyes; Marco A Juárez-Verdayes; Nelson Avonce; Carmen Quinto; Claudia Díaz-Camino; Federico Sanchez
Journal:  Front Plant Sci       Date:  2016-11-01       Impact factor: 5.753
  9 in total

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