Literature DB >> 1980175

Cloning and mapping of the potato virus Y genome and its in vitro expression.

M Karchi1, I Sela, O Edelbaum, A Ori, B Raccah, A Rosner, Y Stram.   

Abstract

Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

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Year:  1990        PMID： 1980175     DOI： 10.1007/bf00265631

Source DB:  PubMed          Journal:  Virus Genes        ISSN： 0920-8569            Impact factor:   2.332


  15 in total

1.  The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein.

Authors:  R Allison; R E Johnston; W G Dougherty
Journal:  Virology       Date:  1986-10-15       Impact factor: 3.616

2.  Nucleotide sequence of potato virus Y (N Strain) genomic RNA.

Authors:  C Robaglia; M Durand-Tardif; M Tronchet; G Boudazin; S Astier-Manifacier; F Casse-Delbart
Journal:  J Gen Virol       Date:  1989-04       Impact factor: 3.891

3.  The nucleotide sequence of tobacco vein mottling virus RNA.

Authors:  L L Domier; K M Franklin; M Shahabuddin; G M Hellmann; J H Overmeyer; S T Hiremath; M F Siaw; G P Lomonossoff; J G Shaw; R E Rhoads
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase.

Authors:  J C Carrington; S M Cary; W G Dougherty
Journal:  J Virol       Date:  1988-07       Impact factor: 5.103

6.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

7.  In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-encoded protease.

Authors:  G M Hellmann; J G Shaw; R E Rhoads
Journal:  Virology       Date:  1988-04       Impact factor: 3.616

8.  Cell-free translation of tobacco vein mottling virus RNA. II. Immunoprecipitation of products by antisera to cylindrical inclusion, nuclear inclusion, and helper component proteins.

Authors:  G M Hellmann; D W Thornbury; E Hiebert; J G Shaw; T P Pirone; R E Rhoads
Journal:  Virology       Date:  1983-01-30       Impact factor: 3.616

9.  A second proteinase encoded by a plant potyvirus genome.

Authors:  J C Carrington; S M Cary; T D Parks; W G Dougherty
Journal:  EMBO J       Date:  1989-02       Impact factor: 11.598

10.  Biochemical and mutational analysis of a plant virus polyprotein cleavage site.

Authors:  W G Dougherty; J C Carrington; S M Cary; T D Parks
Journal:  EMBO J       Date:  1988-05       Impact factor: 11.598
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  2 in total

1.  Expression of the "helper component" protein of potato virus Y (PVY) in E. coli: possible involvement of a third protease.

Authors:  Y Stram; A Chetsrony; H Karchi; M Karchi; O Edelbaum; E Vardi; O Livneh; I Sela
Journal:  Virus Genes       Date:  1993-06       Impact factor: 2.332

2.  Plants transformed with a cistron of a potato virus Y protease (NIa) are resistant to virus infection.

Authors:  E Vardi; I Sela; O Edelbaum; O Livneh; L Kuznetsova; Y Stram
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-15       Impact factor: 11.205
  2 in total

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