Brenda P F A Gomes1, Frederico C Martinho, Morgana E Vianna. 1. Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. bpgomes@fop.unicamp.br
Abstract
INTRODUCTION: This clinical study was conducted to compare the efficacy of chemomechanical preparation with 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) gel on eliminating oral bacterial lipopolysaccharide (LPS) in teeth with pulp necrosis and apical periodontitis. METHODS:Fifty-four root canals were selected. Samples were collect before (s1) and after chemomechanical preparation (s2). Teeth were randomly divided into groups: GI, 2.5% NaOCl (n = 27) and GII, CHX gel (n = 27). Limulus amebocyte lysate assay was used to quantify endotoxins. RESULTS:Endotoxin was present in 100% of the samples investigated, with a median value of 272 endotoxin units (EU)/mL (GI) and of 152.46 EU/mL (GII). As a result of chemomechanical preparation, LPS content was reduced to a median value of 86 EU/mL (GI) and 85 EU/mL (GII). Higher percentage value of endotoxin reduction was found in GI (P < .05). CONCLUSIONS: The 2.5% NaOCl and 2% CHX gel were not effective in eliminating endotoxin from the primarily infected root canals.
RCT Entities:
INTRODUCTION: This clinical study was conducted to compare the efficacy of chemomechanical preparation with 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) gel on eliminating oral bacterial lipopolysaccharide (LPS) in teeth with pulp necrosis and apical periodontitis. METHODS: Fifty-four root canals were selected. Samples were collect before (s1) and after chemomechanical preparation (s2). Teeth were randomly divided into groups: GI, 2.5% NaOCl (n = 27) and GII, CHX gel (n = 27). Limulus amebocyte lysate assay was used to quantify endotoxins. RESULTS: Endotoxin was present in 100% of the samples investigated, with a median value of 272 endotoxin units (EU)/mL (GI) and of 152.46 EU/mL (GII). As a result of chemomechanical preparation, LPS content was reduced to a median value of 86 EU/mL (GI) and 85 EU/mL (GII). Higher percentage value of endotoxin reduction was found in GI (P < .05). CONCLUSIONS: The 2.5% NaOCl and 2% CHX gel were not effective in eliminating endotoxin from the primarily infected root canals.
Authors: M S Endo; F C Martinho; A A Zaia; C C R Ferraz; J F A Almeida; B P F A Gomes Journal: Eur J Clin Microbiol Infect Dis Date: 2012-05-08 Impact factor: 3.267
Authors: Frederico C Martinho; Cinthya C Gomes; Gustavo G Nascimento; Ana P M Gomes; Fábio R M Leite Journal: Clin Oral Investig Date: 2017-06-06 Impact factor: 3.573
Authors: Frederico C Martinho; Fábio R M Leite; Gustavo G Nascimento; Joni A Cirelli; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2014-02-13 Impact factor: 3.573
Authors: Ezequiel S Gabrielli; Augusto R Lima; Priscila A Francisco; Daniel R Herrera; Adriana de-Jesus-Soares; Caio C R Ferraz; José Flávio A Almeida; Marina A Marciano; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2021-06-11 Impact factor: 3.573